Journal article
Mapping and isolation of large peptide fragments from bovine neurophysins and biosynthetic neurophysin-containing species by high-performance liquid chromatography
Analytical biochemistry, v 107(1), pp 11-16
1980
PMID: 7435946
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A peptide mapping procedure suitable for rapid analysis and peptide recovery was devised for the neurophysins. Tryptic fragments of performic acid-oxidized bovine neurophysins I and II were fractionated by reverse-phase high-performance liquid chromatography using γ-cyanopropyl-bonded columns. Elutions were achieved using a gradient from triethylammonium phosphate buffer to mixtures of this buffer with increasing proportions of acetonitrile. All tryptic fragments, except for dipeptides, were separated. Assignments of eluted peaks to particular authentic neurophysin peptides were achieved by collection of peaks and determination of their amino acid compositions. The use of this peptide mapping procedure to detect subpicomole amounts of neurophysin peptides in cell-free biosynthetic products was demonstrated.
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Details
- Title
- Mapping and isolation of large peptide fragments from bovine neurophysins and biosynthetic neurophysin-containing species by high-performance liquid chromatography
- Creators
- Irwin M. Chaiken - National Institute of Arthritis and Musculoskeletal and Skin DiseasesChristopher J. Hough - National Institute of Arthritis and Musculoskeletal and Skin Diseases
- Publication Details
- Analytical biochemistry, v 107(1), pp 11-16
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Drexel University
- Web of Science ID
- WOS:A1980KL77700002
- Scopus ID
- 2-s2.0-0019165616
- Other Identifier
- 991019520530804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Chemistry, Analytical