Journal article
Mapping the genetic region coding for herpes simplex virus resistance to mouse interferon alpha / beta
Journal of general virology, v 74(11), pp 2325-2332
01 Nov 1993
PMID: 8245849
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Herpes simplex virus (HSV) ocular virulence has been associated with strain sensitivity to mouse interferon (IFN)- alpha / beta . To identify the region of the virus genome associated with heightened resistance to this cytokine, intertypic recombinants were constructed using the intact genome of avirulent, IFN-sensitive HSV type 1 (strain 35) and XbaI-digested DNA from virulent, IFN-resistant HSV type 2 (strain 186). An intertypic recombinant, designated HSV-R4, was isolated which grew to titres 10- to 100-fold higher than HSV-1(35) in mouse ocular tissue in vivo, and induced stromal keratitis. The recombinant which was several orders of magnitude more resistant to mouse IFN- alpha / beta than HSV-1(35) had a genome composed of HSV-1(35) DNA except for a 12 kb fragment (0.15 to 0.23 map units) derived from HSV-2(186). To define the IFN resistance locus further, three overlapping subclones of this 12 kb fragment were constructed from the HSV-2(186) genome and subjected to marker rescue experiments. The cloned BamHI D fragment was the only subclone that promoted HSV-1(35) ocular growth in vivo. An intertypic recombinant, designated HSV-R(BD), was isolated from the 35 x 186 BamHI D transfection progeny pool. This recombinant, in contrast to HSV-1(35), was several orders of magnitude more resistant to mouse IFN- alpha / beta inhibition in vitro, grew 10- to 100-fold better in mouse ocular tissue in vivo, and caused severe necrotizing stromal keratitis in BALB/c mice. Analysis of the recombinant genome indicated that the HSV-2 genetic information responsible for IFN resistance of HSV-R(BD) was located within the BamHI D fragment, most likely mapping to that region containing three partial open reading frames designated UL14, UL15 and UL16. The products encoded by this region remain to be identified.
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Details
- Title
- Mapping the genetic region coding for herpes simplex virus resistance to mouse interferon alpha / beta
- Creators
- Ying-Hsiu SuJ E Oakes - University of South AlabamaR Lausch - University of South Alabama
- Publication Details
- Journal of general virology, v 74(11), pp 2325-2332
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1993MF24400003
- Scopus ID
- 2-s2.0-0027383860
- Other Identifier
- 991021463435404721
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- Web of Science research areas
- Biotechnology & Applied Microbiology
- Virology