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Journal article
Marrow stromal cells as a source of progenitor cells for nonhematopoietic tissues in transgenic mice with a phenotype of osteogenesis imperfecta
Proceedings of the National Academy of Sciences - PNAS, Vol.95(3), pp.1142-1147
03 Feb 1998
PMID: 9448299
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Marrow stromal cells from wild-type mice were infused into transgenic mice that had a phenotype of fragile bones resembling osteogenesis imperfecta because they expressed a human minigene for type I collagen. In mice that were irradiated with potentially lethal levels (700 cGy) or sublethal levels (350 cGy), DNA from the donor marrow stromal cells was detected consistently in marrow, bone, cartilage, and lung either 1 or 2.5 mo after the infusions. The DNA also was detected but less frequently in the spleen, brain, and skin. There was a small but statistically significant increase in both collagen content and mineral content of bone 1 mo after the infusion. Similar results were obtained with infusion of relatively large amounts of wild-type whole marrow cells into the transgenic mice. In experiments in which male marrow stromal cells were infused into a female osteogenesis imperfecta-transgenic mouse, fluorescense in situ hybridization assays for the Y chromosome indicated that, after 2.5 mo, donor male cells accounted for 4–19% of the fibroblasts or fibroblast-like cells obtained in primary cultures of the lung, calvaria, cartilage, long bone, tail, and skin. In a parallel experiment in which whole marrow cells from a male mouse were infused into a female immunodeficient rag-2 mouse, donor male cells accounted for 4–6% of the fibroblasts or fibroblast-like cells in primary cultures. The results support previous suggestions that marrow stromal cells or related cells in marrow serve as a source for continual renewal of cells in a number of nonhematopoietic tissues.
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Details
- Title
- Marrow stromal cells as a source of progenitor cells for nonhematopoietic tissues in transgenic mice with a phenotype of osteogenesis imperfecta
- Creators
- Ruth F. Pereira - Drexel University, Allegheny University of the Health Sciences (1996-1998)Michael D. O’Hara - Thomas Jefferson UniversityAlexey V. Laptev - Drexel University, Allegheny University of the Health Sciences (1996-1998)Kenneth W. Halford - Drexel University, Allegheny University of the Health Sciences (1996-1998)Marea D. PollardReiner Class - Drexel University, Allegheny University of the Health Sciences (1996-1998)Daniela Simon - Thomas Jefferson UniversityKristin Livezey - Drexel University, Allegheny University of the Health Sciences (1996-1998)Darwin J. Prockop - Drexel University, Allegheny University of the Health Sciences (1996-1998)
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.95(3), pp.1142-1147
- Publisher
- The National Academy of Sciences
- Number of pages
- 6
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Radiation Oncology (and Nuclear Medicine)
- Web of Science ID
- WOS:000071878500062
- Scopus ID
- 2-s2.0-0032477883
- Other Identifier
- 991019167738904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Hematology