Journal article
Mass action kinetics of vesicle aggregation and fusion
Colloids and surfaces, v 6(4), pp 333-363
01 Jan 1983
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Abstract
The kinetics of Ca
2+-induced fusion of both large and small phosphatidylserine vesicles has been examined theoretically and experimentally. With the Tb/DPA assay for mixing of vesicle contents, the kinetics of vesicle aggregation can be separated from the kinetics of vesicle fusion by varying the initial concentration of vesicles. Both the rate constant for vesicle dimerization and the rate constant for the actual membrane fusion can be determined by this analysis. The kinetics of release of vesicle contents during and following the fusion event have also been examined. The theoretical analysis can be easily adapted to other fusion assays and, as such, provides a unique opportunity for a quantitative evaluation of the factors which affect the fusion of vesicles such as temperature, cations bound to the lipids, and the membrane composition. The effect of aggregation reversibility on the kinetics of the overall fusion process is considered in detail. Analysis of the initial kinetics of the Ca
2+-induced fusion of phosphatidylserine vesicles shows that the fusion step itself can be very rapid (of the order of ms—s) and the initial leakage associated with the fusion event, per se, is essentially zero for the large vesicles and quite small for the small vesicles.
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Details
- Title
- Mass action kinetics of vesicle aggregation and fusion
- Creators
- Joe Bentz - University of California, San FranciscoShlomo Nir - Hebrew University of JerusalemJan Wilschut - University of Groningen
- Publication Details
- Colloids and surfaces, v 6(4), pp 333-363
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:A1983QQ16400005
- Scopus ID
- 2-s2.0-0020127910
- Other Identifier
- 991021463687504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Physical