Journal article
Maternal and postweaning high-fat diets disturb hippocampal gene expression, learning, and memory function
American journal of physiology. Regulatory, integrative and comparative physiology, v 306(8), pp R527-R537
01 Apr 2014
PMID: 24523341
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Page KC, Jones EK, Anday EK. Maternal and postweaning high-fat diets disturb hippocampal gene expression, learning, and memory function. Am J Physiol Regul Integr Comp Physiol 306: R527-R537, 2014. First published February 12, 2014; doi: 10.1152/ajpregu. 00319.2013.-We tested the hypothesis that excess saturated fat consumption during pregnancy, lactation, and/or postweaning alters the expression of genes mediating hippocampal synaptic efficacy and impairs spatial learning and memory in adulthood. Dams were fed control chow or a diet high in saturated fat before mating, during pregnancy, and into lactation. Offspring were weaned to either standard chow or a diet high in saturated fat. The Morris Water Maze was used to evaluate spatial learning and memory. Open field testing was used to evaluate motor activity. Hippocampal gene expression in adult males was measured using RT-PCR and ELISA. Offspring from high fat-fed dams took longer, swam farther, and faster to try and find the hidden platform during the 5-day learning period. Control offspring consuming standard chow spent the most time in memory quadrant during the probe test. Offspring from high fat-fed dams consuming excess saturated fat spent the least. The levels of mRNA and protein for brain-derived neurotrophic factor and activity-regulated cytoskeletal-associated protein were significantly decreased by maternal diet effects. Nerve growth factor mRNA and protein levels were significantly reduced in response to both maternal and postweaning high-fat diets. Expression levels for the N-methyl-D-aspartate receptor (NMDA) receptor subunit NR2B as well as synaptophysin were significantly decreased in response to both maternal and postweaning diets. Synaptotagmin was significantly increased in offspring from high fat-fed dams. These data support the hypothesis that exposure to excess saturated fat during hippocampal development is associated with complex patterns of gene expression and deficits in learning and memory.
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Details
- Title
- Maternal and postweaning high-fat diets disturb hippocampal gene expression, learning, and memory function
- Creators
- Kathleen C. Page - Bucknell UniversityElizabeth K. Jones - Bucknell UniversityEndla K. Anday - Drexel University
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, v 306(8), pp R527-R537
- Publisher
- Amer Physiological Soc
- Number of pages
- 11
- Grant note
- Creight Foundation,Drexel University (Philadelphia, PA).
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:000334679100002
- Scopus ID
- 2-s2.0-84900545301
- Other Identifier
- 991019186805004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Physiology