Journal article
Mathematically engineered stromal cell-derived factor-1 alpha stem cell cytokine analog enhances mechanical properties of infarcted myocardium
The Journal of thoracic and cardiovascular surgery, v 145(1), pp 278-284
01 Jan 2013
PMID: 23244259
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Objective: The biomechanical response to a myocardial infarction consists of ventricular remodeling that leads to dilatation, loss of contractile function, abnormal stress patterns, and ultimately heart failure. We hypothesized that intramyocardial injection of our previously designed pro-angiogenic chemokine, an engineered stromal cell-derived factor-1 alpha analog (ESA), improves mechanical properties of the heart after infarction.
Methods: Male rats (n = 54) underwent either sham surgery (n = 17) with no coronary artery ligation or ligation of the left anterior descending artery (n = 37). The rats in the myocardial infarction group were then randomized to receive either saline (0.1 mL, n = 18) or ESA (6 mu g/kg, n = 19) injected into the myocardium at 4 predetermined spots around the border zone. Echocardiograms were performed preoperatively and before the terminal surgery. After 4 weeks, the hearts were explanted and longitudinally sectioned. Uniaxial tensile testing was completed using an Instron 5543 Microtester. Optical strain was evaluated using custom image acquisition software, Digi-Velpo, and analyzed in MATLAB.
Results: Compared with the saline control group at 4 weeks, the ESA-injected hearts had a greater ejection fraction (71.8% +/- 9.0% vs 55.3% +/- 12.6%, P = .0004), smaller end-diastolic left ventricular internal dimension (0.686 +/- 0.110 cm vs 0.763 +/- 0.160 cm, P = .04), greater cardiac output (36 +/- 11.6 mL/min vs 26.9 +/- 7.3 mL/min, P = .05), and a lower tensile modulus (251 +/- 56 kPa vs 301 +/- 81 kPa, P = .04). The tensile modulus for the sham group was 195 +/- 56 kPa, indicating ESA injection results in a less stiff ventricle.
Conclusions: Direct injection of ESA alters the biomechanical response to myocardial infarction, improving the mechanical properties in the postinfarct heart. (J Thorac Cardiovasc Surg 2013;145:278-84)
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Details
- Title
- Mathematically engineered stromal cell-derived factor-1 alpha stem cell cytokine analog enhances mechanical properties of infarcted myocardium
- Creators
- John W. MacArthur - University of PennsylvaniaAlen Trubelja - University of PennsylvaniaYasuhiro Shudo - University of PennsylvaniaPhilip Hsiao - University of PennsylvaniaAlexander S. Fairman - University of PennsylvaniaElaine Yang - University of PennsylvaniaWilliam Hiesinger - University of PennsylvaniaJoseph J. Sarver - University of PennsylvaniaPavan Atluri - University of PennsylvaniaY. Joseph Woo - University of Pennsylvania
- Publication Details
- The Journal of thoracic and cardiovascular surgery, v 145(1), pp 278-284
- Publisher
- Elsevier
- Number of pages
- 7
- Grant note
- 12POST11620024 / American Heart Association 1R01HL089315-01; T32 HL007843-15 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA T32HL007843 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000312386300047
- Scopus ID
- 2-s2.0-84871242733
- Other Identifier
- 991019176648104721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cardiac & Cardiovascular Systems
- Respiratory System
- Surgery