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Journal article
MaxiK Channel Interactome Reveals its Interaction with GABA Transporter 3 and Heat Shock Protein 60 in the Mammalian Brain
Neuroscience, v 317, pp 76-107
07 Jan 2016
PMID: 26772433
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Large conductance voltage and calcium-activated potassium (MaxiK) channels are activated by membrane depolarization and elevated cytosolic Ca
2+
. In the brain, they localize to neurons and astrocytes, where they play roles such as resetting the membrane potential during an action potential, neurotransmitter release, and neurovascular coupling. MaxiK channels are known to associate with several modulatory proteins and accessory subunits, and each of these interactions can have distinct physiological consequences. To uncover new players in MaxiK channel brain physiology, we applied a directed proteomic approach and obtained MaxiK channel pore-forming α subunit brain interactome using specific antibodies. Controls included immunoprecipitations with rabbit IgG and with anti-MaxiK antibodies in wild type and MaxiK channel knockout mice (
Kcnma1
−/−
), respectively. We have found known and unreported interactive partners that localize to the plasma membrane, extracellular space, cytosol and intracellular organelles including mitochondria, nucleus, endoplasmic reticulum and Golgi apparatus. Localization of MaxiK channel to mitochondria was further confirmed using purified brain mitochondria colabeled with MitoTracker. Independent proof of MaxiK channel interaction with previously unidentified partners is given for GABA transporter 3 (GAT3) and heat shock protein 60 (HSP60). In HEK293T cells, both GAT3 and HSP60 coimmunoprecipitated and colocalized with MaxiK channel; colabeling was observed mainly at the cell periphery with GAT3 and intracellularly with HSP60 with protein proximity indices of ~0.6 and ~0.4, respectively. In rat primary hippocampal neurons, colocalization index was identical for GAT3 (~0.6) and slightly higher for HSP60 (~0.5) association with MaxiK channel. The results of this study provide a complete interactome of MaxiK channel the mouse brain, further establish the localization of MaxiK channel in the mouse brain mitochondria and demonstrate the interaction of MaxiK channel with GAT3 and HSP60 in neurons. The interaction of MaxiK channel with GAT3 opens the possibility of a role of MaxiK channel in GABA homeostasis and signaling.
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Details
- Title
- MaxiK Channel Interactome Reveals its Interaction with GABA Transporter 3 and Heat Shock Protein 60 in the Mammalian Brain
- Creators
- Harpreet Singh - University of California, Los AngelesMin Li - University of California, Los AngelesLyra Hall - Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia PA 19102, USA Department of Anesthesiology, University of California, Los Angeles, CA 90095, USA Department of Neurobiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA Department of Physiology, University of Maryland, Baltimore MD 21201, USA Department of Physiology, University of California, Los Angeles, CA 90095, USA Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA Brain Research Institute, University of California, Los Angeles, CA 90095, USA Cardiovascular Research Laboratory, University of California, Los Angeles, CA 90095, USAScarlett Chen - University of California, Los AngelesSowmya Sukur - Drexel UniversityRong Lu - University of California, Los AngelesAnna Caputo - University of California, Los AngelesAndrea L. Meredith - University of Maryland, BaltimoreEnrico Stefani - University of California, Los AngelesLigia Toro - University of California, Los AngelesSongming Chen - Microbiology and Immunology
- Publication Details
- Neuroscience, v 317, pp 76-107
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000368909800007
- Scopus ID
- 2-s2.0-84955057218
- Other Identifier
- 991019167710204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences