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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Measurement and Analysis of in vitro Actin Polymerization
Methods in molecular biology (Clifton, N.J.), v 1046, pp 273-293
01 Jan 2013
PMCID: PMC3995334
PMID: 23868594
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The polymerization of actin underlies force generation in numerous cellular processes. While actin polymerization can occur spontaneously, cells maintain control over this important process by preventing actin filament nucleation and then allowing stimulated polymerization and elongation by several regulated factors. Actin polymerization, regulated nucleation and controlled elongation activities can be reconstituted
in vitro
, and used to probe the signaling cascades cells use to control when and where actin polymerization occurs. Introducing a pyrene fluorophore allows detection of filament formation by an increase in pyrene fluorescence. This method has been used for many years and continues to be broadly used, owing to its simplicity and flexibility. Here we describe how to perform and analyze these
in vitro
actin polymerization assays, with an emphasis on extracting useful descriptive parameters from kinetic data.
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Details
- Title
- Measurement and Analysis of in vitro Actin Polymerization
- Creators
- Lynda K. Doolittle - Howard Hughes Medical InstituteMichael K. Rosen - Howard Hughes Medical InstituteShae B. Padrick - Howard Hughes Medical Institute
- Publication Details
- Methods in molecular biology (Clifton, N.J.), v 1046, pp 273-293
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000325641400017
- Scopus ID
- 2-s2.0-84934440885
- Other Identifier
- 1627035389; 9781627035385; 991020836329704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology