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Measuring Process Dynamics and Nuclear Migration for Clones of Neural Progenitor Cells
Journal article   Open access   Peer reviewed

Measuring Process Dynamics and Nuclear Migration for Clones of Neural Progenitor Cells

Edgar Cardenas De La Hoz, Mark R. Winter, Maria Apostolopoulou, Sally Temple and Andrew R. Cohen
Computer vision - ECCV ... : ... European Conference on Computer Vision : proceedings. European Conference on Computer Vision, v 9913, pp 291-305
18 Sep 2016
DOI: https://doi.org/10.1007/978-3-319-46604-0_21
PMID: 27878138
url
https://europepmc.org/articles/pmc5116388View
Accepted (AM)Open Access (License Unspecified),  Open

Abstract

in-terkinetic nuclear migration lineaging neural progenitor cells Neural stem cells segmentation stem cell process dynamics stem cell processes tracking
Neural stem and progenitor cells (NPCs) generate processes that extend from the cell body in a dynamic manner. The NPC nucleus migrates along these processes with patterns believed to be tightly coupled to mechanisms of cell cycle regulation and cell fate determination. Here, we describe a new segmentation and tracking approach that allows NPC processes and nuclei to be reliably tracked across multiple rounds of cell division in phase-contrast microscopy images. Results are presented for mouse adult and embryonic NPCs from hundreds of clones, or lineage trees, containing tens of thousands of cells and millions of segmentations. New visualization approaches allow the NPC nuclear and process features to be effectively visualized for an entire clone. Significant differences in process and nuclear dynamics were found among type A and type C adult NPCs, and also between embryonic NPCs cultured from the anterior and posterior cerebral cortex.

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