Journal article
Measuring bacterial growth by tapered fiber and changes in evanescent field
Biosensors & bioelectronics, v 21(7), pp 1339-1344
2006
PMID: 15913977
Abstract
Single mode continuous tapered fibers were fabricated with waist diameters of 6–8
μm and of 11
mm waist lengths. The tapered surface was coated with poly-
l-lysine and
Escherichia coli (
E. coli) (JM 101) expressing green fluorescent protein was immobilized. Growth of this culture at 22 and 32
°C was monitored by 480
nm light transmission through the tapered fiber. Change in transmission is a measure of change in absorption of the evanescent field. The transmission decreased exponentially with cell growth on the tapered surface. Growth rate was determined and compared favorably with cells grown on the same medium in multiwell plates. Significance of the results is that a tapered fiber sensor can be used effectively for rapid assessment to determine the presence of bacteria by growth.
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Details
- Title
- Measuring bacterial growth by tapered fiber and changes in evanescent field
- Creators
- David Maraldo - Department of Chemical Engineering, Drexel University, 32nd and Chestnut Sts, Philadelphia, PA 19104, USAP. Mohana Shankar - Department of Electrical and Computer Engineering, Drexel University, Philadelphia, PA 19104, USARaj Mutharasan - Department of Chemical Engineering, Drexel University, 32nd and Chestnut Sts, Philadelphia, PA 19104, USA
- Publication Details
- Biosensors & bioelectronics, v 21(7), pp 1339-1344
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering; Chemical and Biological Engineering
- Web of Science ID
- WOS:000234644400043
- Scopus ID
- 2-s2.0-29144490189
- Other Identifier
- 991014877794604721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology