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Measuring the dynamic mechanical response of hydrated mouse bone by nanoindentation
Journal article   Open access   Peer reviewed

Measuring the dynamic mechanical response of hydrated mouse bone by nanoindentation

Siddhartha Pathak, J. Gregory Swadener, Surya R Kalidindi, Hayden-William Courtland, Karl J Jepsen and Haviva M Goldman
Journal of the mechanical behavior of biomedical materials, v 4(1), pp 34-43
Jan 2011
PMID: 21094478
url
https://doi.org/10.1016/j.jmbbm.2010.09.002View
Published, Version of Record (VoR) Open

Abstract

Viscoelasticity Nanoindentation Bone
This study demonstrates a novel approach to characterizing hydrated bone’s viscoelastic behavior at the lamellar length scales using dynamic indentation techniques. We studied the submicron-level viscoelastic response of bone tissue from two different inbred mouse strains, A/J and B6, with known differences in whole bone and tissue-level mechanical properties. Our results show that bone having a higher collagen content or a lower mineral-to-matrix ratio demonstrates a trend towards a larger viscoelastic response. When normalized for anatomical location relative to biological growth patterns in the antero-medial (AM) cortex, bone tissue from B6 femora, known to have a lower mineral-to-matrix ratio, is shown to exhibit a significantly higher viscoelastic response compared to A/J tissue. Newer bone regions with a higher collagen content (closer to the endosteal edge of the AM cortex) showed a trend towards a larger viscoelastic response. Our study demonstrates the feasibility of this technique to be used to study local composition-property relationships in bone. Further, this technique of viscoelastic nanoindentation mapping of the bone surface at these submicron length scales is shown to be highly advantageous in studying sub-surface features, such as porosity, of wet hydrated biological specimens, which are difficult to identify using other methods.

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Engineering, Biomedical
Materials Science, Biomaterials
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