Journal article
Mechanically and Chemically Tunable Cell Culture System for Studying the Myofibroblast Phenotype
Langmuir, v 30(19), pp 5481-5487
20 May 2014
PMID: 24787894
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cell culture systems for studying the combined effects of matrix proteins and mechanical forces on the behavior of soft tissue cells have not been well developed. Here, we describe a new biomimetic cell culture system that allows for the study of mixtures of matrix proteins while controlling mechanical stiffness in a range that is physiological for soft tissues. This system consists of layer-by-layer (LbL)-assembled films of native matrix proteins atop mechanically tunable soft supports. We used hepatic stellate cells, which differentiate to myofibroblasts in liver fibrosis, for proof-of-concept studies. By culturing cells on collagen and lumican LbL-modified hydrogels, we demonstrate that this system is noncytotoxic and offers a valid control substrate, that the hydrogel determines the overall system mechanics, and that the addition of lumican to collagen influences the stellate cell phenotype. LbL-modified hydrogels offer the potential to study the influence of complex environmental factors on soft-tissue cells in culture.
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Details
- Title
- Mechanically and Chemically Tunable Cell Culture System for Studying the Myofibroblast Phenotype
- Creators
- Michele K. Saums - University of PennsylvaniaWeifeng Wang - Univ Penn, Sch Engn & Appl Sci, Dept Chem & Biomol Engn, Philadelphia, PA 19104 USABiao Han - Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USALakshmi Madhavan - Univ Penn, Sch Engn & Appl Sci, Dept Chem & Biomol Engn, Philadelphia, PA 19104 USALin Han - Drexel UniversityDaeyeon Lee - Univ Penn, Sch Engn & Appl Sci, Dept Chem & Biomol Engn, Philadelphia, PA 19104 USARebecca G. Wells - University of Pennsylvania
- Publication Details
- Langmuir, v 30(19), pp 5481-5487
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 7
- Grant note
- AGA R01DK058123 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) DK-058123 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Drexel University DMR-1055594 / NSF; National Science Foundation (NSF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000336414500018
- Scopus ID
- 2-s2.0-84901044572
- Other Identifier
- 991019168069404721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary