Journal article
Mechanically dynamic PDMS substrates to investigate changing cell environments
Biomaterials, v 145, pp 23-32
Nov 2017
PMID: 28843064
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Mechanics of the extracellular matrix (ECM) play a pivotal role in governing cell behavior, such as cell spreading and differentiation. ECM mechanics have been recapitulated primarily in elastic hydrogels, including with dynamic properties to mimic complex behaviors (e.g., fibrosis); however, these dynamic hydrogels fail to introduce the viscoelastic nature of many tissues. Here, we developed a two-step crosslinking strategy to first form (via platinum-catalyzed crosslinking) networks of polydimethylsiloxane (PDMS) and then to increase PDMS crosslinking (via thiol-ene click reaction) in a temporally-controlled manner. This photoinitiated reaction increased the compressive modulus of PDMS up to 10-fold within minutes and was conducted under cytocompatible conditions. With stiffening, cells displayed increased spreading, changing from ∼1300 to 1900 μm2 and from ∼2700 to 4600 μm2 for fibroblasts and mesenchymal stem cells, respectively. In addition, higher myofibroblast activation (from ∼2 to 20%) for cardiac fibroblasts was observed with increasing PDMS substrate stiffness. These results indicate a cellular response to changes in PDMS substrate mechanics, along with a demonstration of a mechanically dynamic and photoresponsive PDMS substrate platform to model the dynamic behavior of ECM.
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Details
- Title
- Mechanically dynamic PDMS substrates to investigate changing cell environments
- Creators
- Yi-Cheun Yeh - University of PennsylvaniaElise A. Corbin - University of PennsylvaniaSteven R. Caliari - University of PennsylvaniaLiu Ouyang - Drexel UniversitySebastián L. Vega - University of PennsylvaniaRachel Truitt - University of PennsylvaniaLin Han - Drexel UniversityKenneth B. Margulies - University of PennsylvaniaJason A. Burdick - University of Pennsylvania
- Publication Details
- Biomaterials, v 145, pp 23-32
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000413135200003
- Scopus ID
- 2-s2.0-85028018196
- Other Identifier
- 991019168873304721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials