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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Mediation of Cartilage Matrix Degeneration and Fibrillation by Decorin in Post-traumatic Osteoarthritis
Arthritis & rheumatology (Hoboken, N.J.), v 72(8), pp 1266-1277
Aug 2020
PMID: 32162789
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To elucidate the role of decorin, a small leucine-rich proteoglycan, in the degradation of cartilage matrix during the progression of post-traumatic osteoarthritis (OA).
Three-month-old decorin-null (Dcn
) and inducible decorin-knockout (Dcn
) mice were subjected to surgical destabilization of the medial meniscus (DMM) to induce post-traumatic OA. The OA phenotype that resulted was evaluated by assessing joint morphology and sulfated glycosaminoglycan (sGAG) staining via histological analysis (n = 6 mice per group), surface collagen fibril nanostructure via scanning electron microscopy (n = 4 mice per group), tissue modulus via atomic force microscopy-nanoindentation (n = 5 or more mice per group) and subchondral bone structure via micro-computed tomography (n = 5 mice per group). Femoral head cartilage explants from wild-type and Dcn
mice were stimulated with the inflammatory cytokine interleukin-1β (IL-1β) in vitro (n = 6 mice per group). The resulting chondrocyte response to IL-1β and release of sGAGs were quantified.
In both Dcn
and Dcn
mice, the absence of decorin resulted in accelerated sGAG loss and formation of highly aligned collagen fibrils on the cartilage surface relative to the control (P < 0.05). Also, Dcn
mice developed more salient osteophytes, illustrating more severe OA. In cartilage explants treated with IL-1β, loss of decorin did not alter the expression of either anabolic or catabolic genes. However, a greater proportion of sGAGs was released to the media from Dcn
mouse explants, in both live and devitalized conditions (P < 0.05).
In post-traumatic OA, decorin delays the loss of fragmented aggrecan and fibrillation of cartilage surface, and thus, plays a protective role in ameliorating cartilage degeneration.
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Details
- Title
- Mediation of Cartilage Matrix Degeneration and Fibrillation by Decorin in Post-traumatic Osteoarthritis
- Creators
- Qing Li - Drexel UniversityBiao Han - Drexel University, Philadelphia, PennsylvaniaChao Wang - Drexel UniversityWei Tong - University of PennsylvaniaYulong Wei - University of PennsylvaniaWei-Ju Tseng - University of PennsylvaniaLi-Hsin Han - Drexel UniversityX Sherry Liu - University of PennsylvaniaMotomi Enomoto-Iwamoto - University of Maryland, BaltimoreRobert L Mauck - University of Pennsylvania and Corporal Michael J. Crescenz Department of Veterans Affairs Medical Center, Philadelphia, Pennsylvania.Ling Qin - University of PennsylvaniaRenato V Iozzo - Thomas Jefferson UniversityDavid E Birk - University of South FloridaLin Han - Drexel University
- Publication Details
- Arthritis & rheumatology (Hoboken, N.J.), v 72(8), pp 1266-1277
- Publisher
- Wiley
- Grant note
- P30 AR069619 / NIAMS NIH HHS R01 AR074490 / NIAMS NIH HHS R21 AR066824 / NIAMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000547986800001
- Scopus ID
- 2-s2.0-85086414077
- Other Identifier
- 991019168162704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Rheumatology