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Membrane Receptors Involved in Modulation of Responses of Spinal Dorsal Horn Interneurons Evoked by Feline Group II Muscle Afferents
Journal article   Open access   Peer reviewed

Membrane Receptors Involved in Modulation of Responses of Spinal Dorsal Horn Interneurons Evoked by Feline Group II Muscle Afferents

Kimberly J. Dougherty, B. Anne Bannatyne, Elzbieta Jankowska, Piotr Krutki, David J. Maxwell and Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi
The Journal of neuroscience, v 25(3), pp 584-593
19 Jan 2005
DOI: https://doi.org/10.1523/JNEUROSCI.3797-04.2005
PMID: 15659594
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://www.jneurosci.org/content/jneuro/25/3/584.full.pdfView
Published, Version of Record (VoR) Open
url
https://doi.org/10.1523/JNEUROSCI.3797-04.2005View
Published, Version of Record (VoR) Open

Abstract

Behavioral Cognitive confocal microscopy dorsal horn interneurons group II afferents membrane receptors serotonin spinal cord Systems
Modulatory actions of a metabotropic 5-HT 1A&7 membrane receptor agonist and antagonist [(+/-)-8-hydroxy-2-(di-n-propylamino)-tetralin; N -[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]- N -(2-pyridinyl) cyclohexane-carboxamide] and an ionotropic 5-HT 3 membrane receptor agonist and antagonist [2-methyl-serotonin (2-Me 5-HT); N -(1-azabicyclo[2.2.2]oct-3-yl)-6-chloro-4-methyl-3-oxo-3,4-dihydro-2H-1,4-benzoxazine-8-carboxamide hydrochloride] were investigated on dorsal horn interneurons mediating reflex actions of group II muscle afferents. All drugs were applied ionophoretically in deeply anesthetized cats. Effects of agonists were tested on extracellularly recorded responses of individual interneurons evoked by electrical stimulation of group II afferents in a muscle nerve. Effects of antagonists were tested against the depression of these responses after stimulation of raphe nuclei. The results show that both 5-HT 1A&7 and 5-HT 3 membrane receptors are involved in counteracting the activation of dorsal horn interneurons by group II afferents. Because only quantitative differences were found within the sample of the tested neurons, these results suggest that modulatory actions of 5-HT on excitatory and inhibitory interneurons might be similar. The relationship between 5-HT axons and axons immunoreactive for the 5-HT 3A receptor subunit, which contact dorsal horn interneurons, was analyzed using immunofluorescence and confocal microscopy. Contacts from both types of axons were found on all interneurons, but their distribution and density varied, and there was no obvious relationship between them. In two of six interneurons, 5-HT 3A -immunoreactive axons formed ring-like arrangements around the cell bodies. In previous studies, axons possessing 5-HT 3 receptors were found to be excitatory, and as 2-Me 5-HT depressed transmission to dorsal horn interneurons, the results indicate that 5-HT operates at 5-HT 3 receptors presynaptic to these neurons to depress excitatory transmission.

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