Journal article
Membrane-based, sedimentation-assisted plasma separator for point-of-care applications
Analytical chemistry (Washington), v 85(21), pp 10463-10470
05 Nov 2013
PMID: 24099566
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Often, high sensitivity, point of care, clinical tests, such as HIV viral load, require large volumes of plasma. Although centrifuges are ubiquitously used in clinical laboratories to separate plasma from whole blood, centrifugation is generally inappropriate for on-site testing. Suitable alternatives are not readily available to separate the relatively large volumes of plasma from milliliters of blood that may be needed to meet stringent limit-of-detection specifications for low abundance target molecules. We report on a simple to use, low-cost, pump-free, membrane-based, sedimentation-assisted plasma separator capable of separating a relatively large volume of plasma from undiluted whole blood within minutes. This plasma separator consists of an asymmetric, porous, polysulfone membrane housed in a disposable chamber. The separation process takes advantage of both gravitational sedimentation of blood cells and size exclusion-based filtration. The plasma separator demonstrated a “blood in-plasma out” capability, consistently extracting 275 ±33.5 μL of plasma from 1.8 mL of undiluted whole blood in less than 7 min. The device was used to separate plasma laden with HIV viruses from HIV virus-spiked whole blood with recovery efficiencies of 95.5% ± 3.5%, 88.0% ± 9.5%, and 81.5% ± 12.1% for viral loads of 35,000, 3,500 and 350 copies/mL, respectively. The separation process is self-terminating to prevent excessive hemolysis. The HIV-laden plasma was then injected into our custom-made microfluidic chip for nucleic acid Testing And Was Successfully Subjected To Reverse Transcriptase Loop mediated isothermal amplification (RT-LAMP), demonstrating that the plasma is sufficiently pure to support high efficiency nucleic acid amplification.
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Details
- Title
- Membrane-based, sedimentation-assisted plasma separator for point-of-care applications
- Creators
- Changchun Liu - Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USAMichael Mauk - Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USARobert Gross - Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USAFrederic D Bushman - Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USAPaul H Edelstein - Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USARonald G Collman - Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USAHaim H Bau - Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
- Publication Details
- Analytical chemistry (Washington), v 85(21), pp 10463-10470
- Publisher
- American Chemical Society; Washington, DC
- Grant note
- K25 AI099160 || AI / National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000326711400069
- Scopus ID
- 2-s2.0-84887698431
- Other Identifier
- 991014877694404721
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- Web of Science research areas
- Chemistry, Analytical