Journal article
Mesenspheres of neural crest-derived cells enriched from bone marrow stromal cell subpopulation
Neuroscience letters, v 532(1), pp 70-75
04 Jan 2013
PMID: 23127856
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
► The EGF/FGF2-responsive BMSC subpopulation (BMSC-C2) showed a significant sphere-forming ability. ► BMSC-C2-derived mesenspheres displayed a NCC-like phenotype. ► BMSC-C2-derived mesenspheres were self-renewable and able to differentiate toward neural lineages.
Neural crest-derived cells (NCCs) can be used for cell replacement therapy of neurodegenerative diseases and nerve injury, and it is of significance to open readily accessible tissue sources for NCCs due to their insufficient supply. In this study, we aimed to examine the possibility of enriching NCCs from bone marrow stromal cell (BMSC) subpopulation. The epidermal growth factor/fibroblast growth factor-2 (EGF/FGF2)-responsive BMSC subpopulation (BMSC-C2) was isolated from rat bone marrow by repetitive two-step condition culture. The BMSC-C2 subpopulation showed a long-term proliferative capacity and high cell growth rate, and possessed a significant sphere-forming ability. The mesenspheres derived from BMSC-C2 subpopulation were self-renewable and could express NCC markers, such as CD29, CD44, nestin, CD133 and p75NTR. In particular, the mesenspheres could be induced to differentiate into neuron- and glia-like cells in vitro. Collectively, our results might provide a basis for in-depth studies of recruiting postmigratory NCCs from bone marrow and various neural crest-derived tissues.
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Details
- Title
- Mesenspheres of neural crest-derived cells enriched from bone marrow stromal cell subpopulation
- Creators
- Haiyan Shi - School of Biology and Basic Medical Sciences, Soochow University, Suzhou, JS 215123, ChinaTianyi Zhang - Nantong UniversityLiang Qiang - Nantong UniversityLili Man - Nantong UniversityYuntian Shen - Nantong UniversityFei Ding - Nantong University
- Publication Details
- Neuroscience letters, v 532(1), pp 70-75
- Publisher
- Elsevier Ireland Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000313936700014
- Scopus ID
- 2-s2.0-84871063060
- Other Identifier
- 991019201511304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences