Journal article
Metabolic Labeling to Probe the Spatiotemporal Accumulation of Matrix at the Chondrocyte-Hydrogel Interface
Advanced functional materials, v 30(44)
28 Oct 2020
PMID: 34211359
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Hydrogels are engineered with biochemical and biophysical signals to recreate aspects of the native microenvironment and to control cellular functions such as differentiation and matrix deposition. This deposited matrix accumulates within the pericellular space and likely affects the interactions between encapsulated cells and the engineered hydrogel; however, there has been little work to study the spatiotemporal evolution of matrix at this interface. To address this, metabolic labeling is employed to separately visualize the temporal and spatial positioning of nascent proteins and proteoglycans deposited by chondrocytes. Within covalently crosslinked hyaluronic acid hydrogels, chondrocytes deposit nascent proteins and proteoglycans in the pericellular space within 1 d after encapsulation, and proteoglycans extend further into the hydrogel. The accumulation of this matrix, as measured by an increase in matrix thickness during culture, depends on the initial hydrogel crosslink density with decreased thicknesses for more crosslinked hydrogels. Encapsulated fluorescent beads are used to monitor the hydrogel location and indicate that the emerging nascent matrix physically displaces the hydrogel from the cell membrane with extended culture. These findings suggest that secreted matrix increasingly masks the presentation of engineered hydrogel cues and may have implications for the design of hydrogels in tissue engineering and regenerative medicine.
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Details
- Title
- Metabolic Labeling to Probe the Spatiotemporal Accumulation of Matrix at the Chondrocyte-Hydrogel Interface
- Creators
- Claudia Loebel - University of PennsylvaniaMi Y. Kwon - University of PennsylvaniaChao Wang - Drexel UniversityLin Han - Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, 3141 Chestnut St,Bossone 718, Philadelphia, PA 19104 USARobert L. Mauck - University of PennsylvaniaJason A. Burdick - University of Pennsylvania
- Publication Details
- Advanced functional materials, v 30(44)
- Publisher
- Wiley
- Number of pages
- 10
- Grant note
- 1610525; 1751898 / National Science Foundation; National Science Foundation (NSF) R01 EB008722; T32AR007132 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA CMMI: 15-48571 / Center for Engineering MechanoBiology through the National Science Foundation's STC Program
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000523260400001
- Scopus ID
- 2-s2.0-85082946201
- Other Identifier
- 991019169914404721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology
- Physics, Applied
- Physics, Condensed Matter