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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Metabolic tracing analysis reveals substrate-specific metabolic deficits in platelet storage lesion
Transfusion (Philadelphia, Pa.), v 57(11), pp 2683-2689
Nov 2017
PMID: 28836286
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Storage of platelets (PLTs) results in a progressive defect termed PLT storage lesion (PSL). The PSL is characterized by poor PLT quality on a variety of assays. Metabolic defects are thought to underlie the PSL; thus this study was designed to quantitatively probe specific metabolic pathways over PLT storage.
Relative incorporation of stable isotope-labeled substrates was quantified by isotopologue analysis of key acyl-coenzyme A (CoA) thioester products for fresh, viable (after collection, Days 2-5), and expired PLTs (after Day 5). We examined the incorporation of acetate, glucose, and palmitate into acetyl- and succinyl-CoA via liquid chromatography-tandem mass spectrometry.
Storage-related defects in the incorporation of acetyl-CoA derived from acetate and palmitate were observed. Carbon derived from palmitate and acetate in succinyl-CoA was reduced over storage time. Glucose incorporation into succinyl-CoA increased in viable PLTs and then decreased in expired PLTs. Carbon derived from octanoate and pyruvate remained partially able to incorporate into acetyl- and succinyl-CoA in expired PLTs, with high variability in pyruvate incorporation.
Isotopologue analysis is useful in probing substrate specific defects in the PSL.
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Details
- Title
- Metabolic tracing analysis reveals substrate-specific metabolic deficits in platelet storage lesion
- Creators
- Carrie Sims - Surgical ScienceNoelle Salliant - Surgical ScienceAndrew J Worth - Department of Systems Pharmacology and Translational Therapeutics.Robert Parry - Department of Systems Pharmacology and Translational Therapeutics.Clementina Mesaros - University of PennsylvaniaIan A Blair - University of PennsylvaniaNathaniel W Snyder - Drexel University
- Publication Details
- Transfusion (Philadelphia, Pa.), v 57(11), pp 2683-2689
- Publisher
- Wiley
- Grant note
- P30 ES013508 / NIEHS NIH HHS R03 HD092630 / NICHD NIH HHS R03 CA211820 / NCI NIH HHS K22 ES026235 / NIEHS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- A.J. Drexel Autism Institute
- Web of Science ID
- WOS:000414611800019
- Scopus ID
- 2-s2.0-85032944145
- Other Identifier
- 991019167803404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Hematology