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Metabolism of propionic acid to a novel acyl-coenzyme A thioester by mammalian cell lines and platelets[S]
Journal article   Open access   Peer reviewed

Metabolism of propionic acid to a novel acyl-coenzyme A thioester by mammalian cell lines and platelets[S]

Nathaniel W. Snyder, Sankha S. Basu, Andrew J. Worth, Clementina Mesaros and Ian A. Blair
Journal of lipid research, v 56(1)
01 Jan 2015
DOI: https://doi.org/10.1194/jlr.M055384
PMID: 25424005
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1194/jlr.m055384View
Published, Version of Record (VoR)CC BY V4.0,  Open
url
https://doi.org/10.1194/jlr.M055384View
Published, Version of Record (VoR) Open

Abstract

2-methyl-2-pentenoic acid mass spectrometry propionic acidemia stable isotope labeling by essential nutrients in cell culture stable isotopes
Metabolism of propionate involves the activated acyl-thioester propionyl-CoA intermediate. We employed LC-MS/MS, LC-selected reaction monitoring/MS, and LC-high-resolution MS to investigate metabolism of propionate to acyl-CoA intermediates. We discovered that propionyl-CoA can serve as a precursor to the direct formation of a new six-carbon mono-unsaturated acyl-CoA. Time course and dose-response studies in human hepatocellular carcinoma HepG2 cells demonstrated that the six-carbon mono-unsaturated acyl-CoA was propionate-dependent and underwent further metabolism over time. Studies utilizing [ 13 C 1 ]propionate and [ 13 C 3 ]propionate suggested a mechanism of fatty acid synthesis, which maintained all six-carbon atoms from two propionate molecules. Metabolism of 2,2-[ 2 H 2 ]propionate to the new six-carbon mono-unsaturated acyl-CoA resulted in the complete loss of two deuterium atoms, indicating modification at C2 of the propionyl moiety. Coelution experiments and isotopic tracer studies confirmed that the new acyl-CoA was trans -2-methyl-2-pentenoyl-CoA. Acyl-CoA profiles following treatment of HepG2 cells with mono-unsaturated six-carbon fatty acids also supported this conclusion. Similar results were obtained with human platelets, mouse hepatocellular carcinoma Hepa1c1c7 cells, human bronchoalveolar carcinoma H358 cells, and human colon adenocarcinoma LoVo cells. Interestingly, trans -2-methyl-2-pentenoyl-CoA corresponds to a previously described acylcarnitine tentatively described in patients with propionic and methylmalonic acidemia. We have proposed a mechanism for this metabolic route consistent with all of the above findings.

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14 citations in Scopus

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Collaboration types
Domestic collaboration
Web of Science research areas
Biochemistry & Molecular Biology
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