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Methods of comparative proteomic profiling for disease diagnostics
Journal article   Peer reviewed

Methods of comparative proteomic profiling for disease diagnostics

Laura F Steel, Brian B Haab and Samir M Hanash
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, v 815(1-2), pp 275-284
05 Feb 2005
DOI: https://doi.org/10.1016/j.jchromb.2004.10.072
PMID: 15652816
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Chromatography, Liquid Diagnosis Electrophoresis, Gel, Two-Dimensional Mass Spectrometry Protein Array Analysis Proteins - isolation & purification Proteomics - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
The recent development of numerous technologies for proteome analysis holds the promise of new and more precise methods for disease diagnosis. In this review, we provide an overview of some of these technologies including two-dimensional gel electrophoresis (2DE), historically the workhorse of proteomic analysis, as well as some newer approaches such as liquid phase separations combined with mass spectrometry, and protein microarrays. It is evident that each method has its own strengths and weaknesses and no single method will be optimal in all applications. However, the continuing development of innovative strategies for protein separation and analysis is providing a wealth of new tools for multi-dimensional protein profiling that will advance our capabilities in disease diagnostics and our understanding of disease pathology.

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41 citations in Web of Science
56 citations in Scopus

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Collaboration types
Domestic collaboration
Web of Science research areas
Biochemical Research Methods
Chemistry, Analytical
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