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Microelectrode studies of facilitated O2 transport across hemoglobin and myoglobin layers
Journal article   Peer reviewed

Microelectrode studies of facilitated O2 transport across hemoglobin and myoglobin layers

D G Buerk, L Hoofd and Z Turek
Advances in experimental medicine and biology, v 248, pp 125-135
1989
PMID: 2782139

Abstract

Animals Cattle Electrochemistry - methods Hemoglobins - metabolism Kinetics Microelectrodes Myoglobin - metabolism Oxygen - blood Oxyhemoglobins - metabolism Partial Pressure
Experimentally measured PO2 profiles across layers of hemoglobin and myoglobin solutions were compared with profiles predicted from facilitated transport theory assuming chemical equilibrium. Measurements across myoglobin layers were in excellent agreement with theory, but measurements across hemoglobin layers departed from theory at low PO2. This departure was greatest for salt-free hemoglobin solution, which may be caused by an electrical potential formed by a pH gradient in the layer as oxyhemoglobin is deoxygenated.

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