Journal article
Microfabricated mimics of in vivo structural cues for the study of guided tumor cell migration
Lab on a chip, v 12(21), pp 4424-4432
07 Nov 2012
PMID: 22936003
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Guided cell migration plays a crucial role in tumor metastasis, which is considered to be the major cause of death in cancer patients. Such behavior is regulated in part by micro/nanoscale topographical cues present in the parenchyma or stroma in the form of fiber-like and/or conduit-like structures (e. g., white matter tracts, blood/lymphatic vessels, subpial and subperitoneal spaces). In this paper we used soft lithography micromolding to develop a tissue culture polystyrene platform with a microscale surface pattern that was able to induce guided cell motility along/through fiber-/conduit-like structures. The migratory behaviors of primary (glioma) and metastatic (lung and colon) tumors excised from the brain were monitored via time-lapse microscopy at the single cell level. All the tumor cells exhibited axially persistent cell migration, with percentages of unidirectionally motile cells of 84.0 +/- 3.5%, 58.3 +/- 6.8% and 69.4 +/- 5.4% for the glioma, lung, and colon tumor cells, respectively. Lung tumor cells showed the highest migratory velocities (41.8 +/- 4.6 mu m h(-1)) compared to glioma (24.0 +/- 1.8 mu m h(-1)) and colon (26.7 +/- 2.8 mu m h(-1)) tumor cells. This platform could potentially be used in conjunction with other biological assays to probe the mechanisms underlying the metastatic phenotype under guided cell migration conditions, and possibly by itself as an indicator of the effectiveness of treatments that target specific tumor cell motility behaviors.
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Details
- Title
- Microfabricated mimics of in vivo structural cues for the study of guided tumor cell migration
- Creators
- Daniel Gallego-Perez - The Ohio State UniversityNatalia Higuita-Castro - The Ohio State UniversityLisa Denning - The Ohio State UniversityJessica DeJesus - The Ohio State UniversityKirstin Dahl - The Ohio State UniversityAtom Sarkar - Geisinger Health SystemDerek J. Hansford - The Ohio State University
- Publication Details
- Lab on a chip, v 12(21), pp 4424-4432
- Publisher
- Royal Soc Chemistry
- Number of pages
- 9
- Grant note
- F49620-03-1-0421 / AFOSR MURI; United States Department of Defense; Air Force Office of Scientific Research (AFOSR); MURI EEC-0425626 / National Science Foundation; National Science Foundation (NSF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurology; Neurosurgery
- Web of Science ID
- WOS:000310916100029
- Scopus ID
- 2-s2.0-84867325832
- Other Identifier
- 991021960652104721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- Chemistry, Multidisciplinary
- Instruments & Instrumentation
- Nanoscience & Nanotechnology