Journal article
Microfluidic Synthesis of Biodegradable Polyethylene-Glycol Microspheres for Controlled Delivery of Proteins and DNA Nanoparticles
ACS biomaterials science & engineering, v 1(3), pp 157-165
01 Mar 2015
PMID: 33429514
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Polymeric microspheres represent an injectable platform for controlling the release of a variety of biologics; microspheres may be combined in a modular fashion to achieve temporal release of two or more biomolecules. Microfluidics offers a versatile platform for synthesizing uniform polymeric microspheres harboring a variety of biologics under relatively mild conditions. Poly(ethylene glycol) (PEG) is a bioinert polymer that can be easily tailored to encapsulate and control the release of biologics. In this study, we report the microfluidic synthesis of biodegradable PEG-based microparticles for controlled release of growth factors or DNA nanoparticles. Simple changes in microfluidic design increased the rate of microparticle formation and controlled the size of the microspheres. Mesh size and degradation rate were controlled by varying the PEG polymer weight percent from 7.5 to 15% (w/v), thus tuning the release of growth factors and DNA nanoparticles, which retained their bioactivity in assays of cell proliferation and DNA transfection, respectively. This platform may provide a useful tool for synthesizing microspheres for use as injectable carriers to achieve coordinated growth factor or DNA nanoparticle release in therapeutic applications.
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Details
- Title
- Microfluidic Synthesis of Biodegradable Polyethylene-Glycol Microspheres for Controlled Delivery of Proteins and DNA Nanoparticles
- Creators
- Lorenzo Deveza - Stanford UniversityJothikritika Ashoken - San Jose State UniversityGloria Castaneda - Stanford UniversityXinming Tong - Stanford UniversityMichael Keeney - Stanford UniversityLi-Hsin Han - Stanford UniversityFan Yang - Stanford University
- Publication Details
- ACS biomaterials science & engineering, v 1(3), pp 157-165
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 9
- Grant note
- Stanford Medical Scholars Research Program 10SDG2600001 / American Heart Association (National Scientist Development); American Heart Association
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics; Drexel University
- Web of Science ID
- WOS:000369347200004
- Scopus ID
- 2-s2.0-84969222346
- Other Identifier
- 991020100051404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Materials Science, Biomaterials