Journal article
Microfluidic platform for hepatitis B viral replication study
Biomedical microdevices, v 10(3), pp 393-402
Jun 2008
PMID: 18165913
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Hepatocytes, the cells responsible for the metabolic and detoxification processes in the liver, are the predominant target of hepatitis B virus (HBV) infections, a major cause of liver cancer. The limited availability of normal human hepatocytes for cell-culture based studies is a significant challenge in HBV-associated liver cancer research. Therefore, there is a need for miniaturized cell-culture systems that can serve as a platform for studying the effect of HBV infections on hepatocyte physiology. Here, we present a microfluidic platform that can be used to study HBV replication in both rat and human hepatocytes. Polydimethylsiloxane (PDMS) microchannels fabricated using soft lithography techniques served as a culture vessel for both primary rat hepatocytes (PRH) and a human hepatoblastoma cell line, HepG2. The micro cell-culture chamber was then used as a model for HBV replication studies. Cells were grown in static culture conditions and either transfected with an HBV-genome cDNA or infected with the viral genome expressed from a recombinant adenovirus. Supernatants collected from the microchannels were assayed for secreted HBV using polymerase chain reaction (PCR). We achieved approximately 40 and 10% transfection efficiencies in HepG2 cells and PRH respectively, and 80–100% adenoviral infection efficiency in PRH comparable to standard tissue culture methods. Moreover, we successfully detected replicated HBV using our novel platform. This platform can be easily extended to studies involving DNA transfection or HBV infection of primary human hepatocytes since only a small number of cells are required for studies in microfluidic chambers.
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Details
- Title
- Microfluidic platform for hepatitis B viral replication study
- Creators
- Temitope Sodunke - Department of Mechanical Engineering and Mechanics Drexel University Philadelphia PA 19104 USAMichael Bouchard - Department of Biochemistry and Molecular Biology Drexel University College of Medicine (DUCOM) Philadelphia PA 19102 USAHongseok (Moses) Noh - Department of Mechanical Engineering and Mechanics Drexel University Philadelphia PA 19104 USA
- Publication Details
- Biomedical microdevices, v 10(3), pp 393-402
- Publisher
- Springer US; Boston
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000254900300008
- Scopus ID
- 2-s2.0-42149104439
- Other Identifier
- 991014877761804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Engineering, Biomedical
- Nanoscience & Nanotechnology