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Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens
Journal article   Open access   Peer reviewed

Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens

Elizabeth Wulff-Burchfield, Wiley A. Schell, Allen E. Eckhardt, Michael G. Pollack, Zhishan Hua, Jeremy L. Rouse, Vamsee K. Pamula, Vijay Srinivasan, Jonathan L. Benton, Barbara D. Alexander, …
Diagnostic microbiology and infectious disease, v 67(1), pp 22-29
01 May 2010
DOI: https://doi.org/10.1016/j.diagmicrobio.2009.12.020
PMID: 20227222
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1016/j.diagmicrobio.2009.12.020View
Published, Version of Record (VoR)Open Access (License Unspecified),  Open

Abstract

Community-acquired pneumonia Diagnostic microbiology DNA-based diagnostics Mycoplasma pneumoniae Real-time PCR
Rapid, accurate diagnosis of community-acquired pneumonia (CAP) due to Mycoplasma pneumoniae is compromised by low sensitivity of culture and serology. Polymerase chain reaction (PCR) has emerged as a sensitive method to detect M. pneumoniae DNA in clinical specimens. However, conventional real-time PCR is not cost-effective for routine or outpatient implementation. Here, we evaluate a novel microfluidic real-time PCR platform (Advanced Liquid Logic, Research Triangle Park, NC) that is rapid, portable, and fully automated. We enrolled patients with CAP and extracted DNA from nasopharyngeal wash (NPW) specimens using a biotinylated capture probe and streptavidin-coupled magnetic beads. Each extract was tested for M. pneumoniae-specific DNA by real-time PCR on both conventional and microfluidic platforms using Taqman probe and primers. Three of 59 (5.0%) NPWs were positive, and agreement between the methods was 98%. The microfluidic platform was equally sensitive but 3 times faster and offers an inexpensive and convenient diagnostic test for microbial DNA.

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38 citations in Web of Science
47 citations in Scopus

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Collaboration types
Domestic collaboration
Web of Science research areas
Infectious Diseases
Microbiology
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