Journal article
Microfluidic quantification of multiple enteric and opportunistic bacterial pathogens in roof-harvested rainwater tank samples
Environmental monitoring and assessment, v 190(2), pp 105-11
30 Jan 2018
PMID: 29383497
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Potable and non-potable uses of roof-harvested rainwater (RHRW) are increasing due to water shortages. To protect human health risks, it is important to identify and quantify disease-causing pathogens in RHRW so that appropriate treatment options can be implemented. We used a microfluidic quantitative PCR (MFQPCR) system for the quantitative detection of a wide array of fecal indicator bacteria (FIB) and pathogens in RHRW tank samples along with culturable FIB and conventional qPCR analysis of selected pathogens. Among the nine pathogenic bacteria and their associated genes tested with the MFQPCR, 4.86 and 2.77% samples were positive for Legionella pneumophila and Shigella spp., respectively. The remaining seven pathogens were absent. MFQPCR and conventional qPCR results showed good agreement. Therefore, direct pathogen quantification by MFQPCR systems may be advantageous for circumstances where a thorough microbial analysis is required to assess the public health risks from multiple pathogens that occur simultaneously in the target water source.
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Details
- Title
- Microfluidic quantification of multiple enteric and opportunistic bacterial pathogens in roof-harvested rainwater tank samples
- Creators
- Warish Ahmed - Commonwealth Scientific and Industrial Research OrganisationQian Zhang - Biotechnology InstituteSatoshi Ishii - Biotechnology InstituteKerry Hamilton - Drexel UniversityCharles Haas - Drexel University
- Publication Details
- Environmental monitoring and assessment, v 190(2), pp 105-11
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Civil, Architectural, and Environmental Engineering
- Web of Science ID
- WOS:000424113700051
- Scopus ID
- 2-s2.0-85041295030
- Other Identifier
- 991019168684404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Environmental Sciences