Journal article
Microinterfaces in biopolymer-based bicontinuous hydrogels guide rapid 3D cell migration
Nature communications, v 15(1), pp 2766-2766
29 Mar 2024
PMID: 38553465
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cell migration is critical for tissue development and regeneration but requires extracellular environments that are conducive to motion. Cells may actively generate migratory routes in vivo by degrading or remodeling their environments or instead utilize existing extracellular matrix microstructures or microtracks as innate pathways for migration. While hydrogels in general are valuable tools for probing the extracellular regulators of 3-dimensional migration, few recapitulate these natural migration paths. Here, we develop a biopolymer-based bicontinuous hydrogel system that comprises a covalent hydrogel of enzymatically crosslinked gelatin and a physical hydrogel of guest and host moieties bonded to hyaluronic acid. Bicontinuous hydrogels form through controlled solution immiscibility, and their continuous subdomains and high micro-interfacial surface area enable rapid 3D migration, particularly when compared to homogeneous hydrogels. Migratory behavior is mesenchymal in nature and regulated by biochemical and biophysical signals from the hydrogel, which is shown across various cell types and physiologically relevant contexts (e.g., cell spheroids, ex vivo tissues, in vivo tissues). Our findings introduce a design that leverages important local interfaces to guide rapid cell migration.
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Details
- Title
- Microinterfaces in biopolymer-based bicontinuous hydrogels guide rapid 3D cell migration
- Creators
- Karen L Xu - University of PennsylvaniaNikolas Di Caprio - University of PennsylvaniaHooman Fallahi - Drexel UniversityMohammad Dehghany - University of PennsylvaniaMatthew D Davidson - University of PennsylvaniaLorielle Laforest - University of PennsylvaniaBrian C H Cheung - Cornell UniversityYuqi Zhang - University of PennsylvaniaMingming Wu - Cornell UniversityVivek Shenoy - University of PennsylvaniaLin Han - Drexel UniversityRobert L Mauck - University of PennsylvaniaJason A Burdick - University of Pennsylvania
- Publication Details
- Nature communications, v 15(1), pp 2766-2766
- Publisher
- Springer Nature
- Grant note
- R01 CA221346 / NCI NIH HHS F30 AG074508 / NIA NIH HHS P30 AR069619 / NIAMS NIH HHS R01 AR077362 / NIAMS NIH HHS R01 AR056624 / NIAMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:001195597700024
- Scopus ID
- 2-s2.0-85189042955
- Other Identifier
- 991021865115604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology