Journal article
Micromanipulation of Adhesion of a Jurkat Cell to a Planar Bilayer Membrane Containing Lymphocyte Function-Associated Antigen 3 Molecules
The Journal of cell biology, v 116(4), pp 997-1006
01 Feb 1992
PMID: 1370839
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cell adhesion plays a fundamental role in the organization of cells in differentiated organs, cell motility, and immune response. A novel micromanipulation method is employed to quantify the direct contribution of surface adhesion receptors to the physical strength of cell adhesion. In this technique, a cell is brought into contact with a glass-supported planar membrane reconstituted with a known concentration of a given type of adhesion molecules. After a period of incubation (5-10 min), the cell is detached from the planar bilayer by pulling away the pipette holding the cell in the direction perpendicular to the glass-supported planar bilayer. In particular, we investigated the adhesion between a Jurkat cell expressing CD2 and a glass-supported planar bilayer containing either the glycosyl-phosphatidylinositol (GPI) or the transmembrane (TM) isoform of the counter-receptor lymphocyte function-associated antigen 3 (LFA-3) at a concentration of 1,000 molecules/μ m2. In response to the pipette force the Jurkat cells that adhered to the planar bilayer containing the GPI isoform of LFA-3 underwent extensive elongation. When the contact radius was reduced by ∼50%, the cell then detached quickly from its substrate. The aspiration pressure required to detach a Jurkat cell from its substrate was comparable to that required to detach a cytotoxic T cell from its target cell. Jurkat cells that had been separated from the substrate again adhered strongly to the planar bilayer when brought to proximity by micromanipulation. In experiments using the planar bilayer containing the TM isoform of LFA-3, Jurkat cells detached with little resistance to micromanipulation and without changing their round shape.
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Details
- Title
- Micromanipulation of Adhesion of a Jurkat Cell to a Planar Bilayer Membrane Containing Lymphocyte Function-Associated Antigen 3 Molecules
- Creators
- Aydin Tözeren - Catholic University of AmericaK-L. Paul Sung - Catholic University of AmericaLanping A. Sung - University of AmericaMichael L. Dustin - University of AmericaPo-Ying Chan - University of AmericaTimothy A. Springer - University of AmericaShu Chien - Catholic University of America
- Publication Details
- The Journal of cell biology, v 116(4), pp 997-1006
- Publisher
- Rockefeller University Press
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:A1992HD55000015
- Scopus ID
- 2-s2.0-0026593456
- Other Identifier
- 991021895669504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology