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Micromechanical measurement of membrane receptor binding for label-free drug discovery
Journal article   Peer reviewed

Micromechanical measurement of membrane receptor binding for label-free drug discovery

Yifei Zhang, Srinivasan P Venkatachalan, Hao Xu, Xiaohe Xu, Prasad Joshi, Hai-Feng Ji and Marvin Schulte
Biosensors & bioelectronics, v 19(11), pp 1473-1478
2004
DOI: https://doi.org/10.1016/j.bios.2003.12.011
PMID: 15093219
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Serotonin Micromechanical motion Microcantilever MEMs Drug discovery Label-free 5-HT3
A potential novel binding assay based on binding-driven micromechanical motion is described. A membrane preparation containing 5-HT3AS receptors was used to modify a microcantilever. The modified microcantilever was found to bend on application of the naturally occurring agonist (5-hydroxytryptamine, which is also called serotonin) or the antagonist MDL-72222, but not to other similar molecules. Control experiments show that cantilevers modified by membrane preparations that do not contain 5-HT3AS receptors do not respond to serotonin or MDL-72222. Kd values obtained for serotonin and MDL-72222 are identical to those obtained from radio-ligand binding assays. These results suggest that the microcantilever system has potential for use in label-free, drug screening applications.

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26 citations in Web of Science
27 citations in Scopus

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Web of Science research areas
Biophysics
Biotechnology & Applied Microbiology
Chemistry, Analytical
Electrochemistry
Nanoscience & Nanotechnology
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