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Microscopic and immunohistochemical analyses of the claw of the nesting dinosaur, Citipati osmolskae
Journal article   Open access

Microscopic and immunohistochemical analyses of the claw of the nesting dinosaur, Citipati osmolskae

Alison E Moyer, Wenxia Zheng and Mary H Schweitzer
Proceedings of the Royal Society. B, Biological sciences, v 283(1842), 20161997
16 Nov 2016
PMID: 28120795
url
https://doi.org/10.1098/rspb.2016.1997View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze) Open

Abstract

Animals Biological Evolution Birds - anatomy & histology Dinosaurs - anatomy & histology Feathers Fossils Hoof and Claw - anatomy & histology Keratins - chemistry
One of the most well-recognized Cretaceous fossils is Citipati osmolskae (MPC-D 100/979), an oviraptorid dinosaur discovered in brooding position on a nest of unhatched eggs. The original description refers to a thin lens of white material extending from a manus ungual, which was proposed to represent original keratinous claw sheath that, in life, would have covered it. Here, we test the hypothesis that this exceptional morphological preservation extends to the molecular level. The fossil sheath was compared with that of extant birds, revealing similar morphology and microstructural organization. In living birds, the claw sheath consists primarily of two structural proteins; alpha-keratin, expressed in all vertebrates, and beta-keratin, found only in reptiles and birds (sauropsids). We employed antibodies raised against avian feathers, which comprise almost entirely of beta-keratin, to demonstrate that fossil tissues respond with the same specificity, though less intensity, as those from living birds. Furthermore, we show that calcium chelation greatly increased antibody reactivity, suggesting a role for calcium in the preservation of this fossil material.

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Collaboration types
Domestic collaboration
Web of Science research areas
Biology
Ecology
Evolutionary Biology
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