Journal article
Microtopography and flow modulate the direction of endothelial cell migration
American journal of physiology. Heart and circulatory physiology, v 294(2), pp H1027-H1035
01 Feb 2008
PMID: 18156201
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The migration of vascular endothelial cells under flow can be modulated by the addition of chemical or mechanical stimuli. The aim of this study was to investigate how topographic cues derived from a substrate containing three-dimensional microtopography interact with fluid shear stress in directing endothelial cell migration. Subconfluent bovine aortic endothelial cells were seeded on fibronectin-coated poly(dimethylsiloxane) substrates patterned with a combinatorial array of parallel and orthogonal microgrooves ranging from 2 to 5 mu m in width at a constant depth of 1 mu m. During a 4-h time-lapse observation in the absence of flow, the majority of the prealigned cells migrated parallel to the grooves with the distribution of their focal adhesions (FAs) depending on the groove width. No change in this migratory pattern was observed after the cells were exposed to moderate shear stress (13.5 dyn/cm(2)), irrespective of groove direction with respect to flow. After 4-h exposure to high shear stress (58 dyn/cm(2)) parallel to the grooves, the cells continued to migrate in the direction of both grooves and flow. By contrast, when microgrooves were oriented perpendicular to flow, most cells migrated orthogonal to the grooves and downstream with flow. Despite the change in the migration direction of the cells under high shear stress, most FAs and actin microfilaments maintained their original alignment parallel to the grooves, suggesting that topographic cues were more effective than those derived from shear stress in guiding the orientation of cytoskeletal and adhesion proteins during the initial exposure to flow.
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Details
- Title
- Microtopography and flow modulate the direction of endothelial cell migration
- Creators
- P. Uttayarat - University of PennsylvaniaM. Chen - Univ Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USAM. Li - Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USAF. D. Allen - Drexel UniversityR. J. Composto - Univ Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USAP. I. Lelkes - Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USA
- Publication Details
- American journal of physiology. Heart and circulatory physiology, v 294(2), pp H1027-H1035
- Publisher
- Amer Physiological Soc
- Number of pages
- 9
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000253072400057
- Scopus ID
- 2-s2.0-39149144508
- Other Identifier
- 991019168042204721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cardiac & Cardiovascular Systems
- Peripheral Vascular Disease
- Physiology