Journal article
Microtubule Organization and Stability in the Oligodendrocyte
The Journal of neuroscience, v 17(13), pp 4921-4932
01 Jul 1997
PMID: 9185530
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The oligodendrocyte is the glial cell responsible for the formation and maintenance of CNS myelin. Because the development of neuronal morphology is known to depend on the presence of highly organized microtubule arrays, it may be hypothesized that the properties of microtubules influence the form and function of oligodendrocytes. The goals of the present study were to define the physical attributes of microtubules in oligodendrocytes maintained
in vitro
. The results of electron and confocal microscopy indicate that microtubules are present throughout the cell bodies and large and small processes of oligodendrocytes and are rarely associated with discrete microtubule-organizing centers. A modified “hooking” protocol demonstrated that the polarity orientation of microtubules is uniformly plus-end distal in small oligodendrocyte processes, compared with a nonuniform, predominantly plus-end distal orientation in large processes. Oligodendrocytes were exposed to the microtubule-depolymerizing drug nocodazole to examine microtubule stability in these cells. The results suggest that oligodendrocyte microtubules can be resolved into at least three distinct microtubule populations that differ in their kinetics of depolymerization in the presence of nocodazole. These findings suggest that the properties of the oligodendrocyte microtubule array reflect the functions of the different regions of this highly specialized cell.
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Details
- Title
- Microtubule Organization and Stability in the Oligodendrocyte
- Creators
- Katharine F LunnPeter W BaasIan D Duncan
- Publication Details
- The Journal of neuroscience, v 17(13), pp 4921-4932
- Publisher
- Society for Neuroscience
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1997XE95200002
- Scopus ID
- 2-s2.0-0030915601
- Other Identifier
- 991014878539504721
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- Web of Science research areas
- Neurosciences