Journal article
Microtubule alterations in cultured taiep rat oligodendrocytes lead to deficits in myelin membrane formation
Journal of neurocytology, v 28(8), pp 671-684
Aug 1999
PMID: 10851346
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The taiep rat is a myelin mutant in which hypomyelination and progressive demyelination of the CNS are accompanied by an accumulation of microtubules within oligodendrocytes. To investigate whether and how the myelin defects were caused by microtubule abnormalities, we have established a taiep oligodendrocyte culture system in which mutant cells produce abnormally high levels of tubulin and microtubule-associated proteins and exhibit myelin defects. The studies show that abnormal microtubule accumulation and tight microtubule bundles developed in the taiep oligodendrocytes, with a higher ratio of minus-end-distal to plus-end-distal microtubules in their processes. Initially, in culture, immature taiep oligodendrocytes which have higher levels of tubulin than controls extend roughly twice as much membrane sheet as controls. The membrane sheets of the mature taiep oligodendrocytes which display the microtubule accumulation, however, grew much less rapidly compared to controls. By the fifth day in culture, a majority of the taiep oligodendrocytes had ceased the expansion of their membrane sheets and in some cases the sheets retracted. The levels of the myelin proteins, proteolipid protein and myelin-associated glycoprotein, were also markedly diminished in the mature taiep oligodendrocytes. Treatment with the microtubule depolymerizing drug nocodazole prevented not only the accumulation of microtubules but also restored the normal distribution of proteolipid proteins within the taiep oligodendrocytes. These data demonstrate that myelin synthesis in the oligodendrocyte cultures relies on the formation of a normal microtubule array, and the microtubule abnormalities are directly responsible for the myelin deficit in the taiep oligodendrocytes.
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Details
- Title
- Microtubule alterations in cultured taiep rat oligodendrocytes lead to deficits in myelin membrane formation
- Creators
- J Song - Department of Medical Science School of Veterinary Medicine USAL.T O'connor - Department of Medical Science School of Veterinary Medicine USAW Yu - Department of Anatomy, School of the Medicine University of Wisconsin Madison WI 53706 USAP.W Baas - Department of Anatomy, School of the Medicine University of Wisconsin Madison WI 53706 USAI.D Duncan - Department of Medical Science School of Veterinary Medicine USA
- Publication Details
- Journal of neurocytology, v 28(8), pp 671-684
- Publisher
- Kluwer Academic Publishers; Boston
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000087615000005
- Scopus ID
- 2-s2.0-0033301212
- Other Identifier
- 991014877931204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell Biology
- Neurosciences