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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Mimicking white matter tract topography using core–shell electrospun nanofibers to examine migration of malignant brain tumors
Biomaterials, v 34(21), pp 5181-5190
01 Jul 2013
PMID: 23601662
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Glioblastoma multiforme (GBM), one of the deadliest forms of human cancer, is characterized by its high infiltration capacity, partially regulated by the neural extracellular matrix (ECM). A major limitation in developing effective treatments is the lack of in vitro models that mimic features of GBM migration highways. Ideally, these models would permit tunable control of mechanics and chemistry to allow the unique role of each of these components to be examined. To address this need, we developed aligned nanofiber biomaterials via core–shell electrospinning that permit systematic study of mechanical and chemical influences on cell adhesion and migration. These models mimic the topography of white matter tracts, a major GBM migration ‘highway’. To independently investigate the influence of chemistry and mechanics on GBM behaviors, nanofiber mechanics were modulated by using different polymers (i.e., gelatin, poly(ethersulfone), poly(dimethylsiloxane)) in the ‘core’ while employing a common poly(ε-caprolactone) (PCL) ‘shell’ to conserve surface chemistry. These materials revealed GBM sensitivity to nanofiber mechanics, with single cell morphology (Feret diameter), migration speed, focal adhesion kinase (FAK) and myosin light chain 2 (MLC2) expression all showing a strong dependence on nanofiber modulus. Similarly, modulating nanofiber chemistry using extracellular matrix molecules (i.e., hyaluronic acid (HA), collagen, and Matrigel) in the ‘shell’ material with a common PCL ‘core’ to conserve mechanical properties revealed GBM sensitivity to HA; specifically, a negative effect on migration. This system, which mimics the topographical features of white matter tracts, should allow further examination of the complex interplay of mechanics, chemistry, and topography in regulating brain tumor behaviors.
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Details
- Title
- Mimicking white matter tract topography using core–shell electrospun nanofibers to examine migration of malignant brain tumors
- Creators
- Shreyas S. Rao - The Ohio State UniversityMark T. Nelson - The Ohio State UniversityRuipeng Xue - The Ohio State UniversityJessica K. DeJesus - The Ohio State UniversityMariano S. Viapiano - Neurological SurgeryJohn J. Lannutti - The Ohio State UniversityAtom Sarkar - Geisinger Health SystemJessica O. Winter - The Ohio State University
- Publication Details
- Biomaterials, v 34(21), pp 5181-5190
- Publisher
- Elsevier
- Number of pages
- 10
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurology; Neurosurgery
- Web of Science ID
- WOS:000319630000027
- Scopus ID
- 2-s2.0-84877046417
- Other Identifier
- 991021960654604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials