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Journal article
Mitochondria coordinate sites of axon branching through localized intra-axonal protein synthesis
Cell reports (Cambridge), v 5(6), pp 1564-1575
26 Dec 2013
PMID: 24332852
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The branching of axons is a fundamental aspect of nervous system development and neuroplasticity. We report that branching of sensory axons in the presence of nerve growth factor (NGF) occurs at sites populated by stalled mitochondria. Translational machinery targets to presumptive branching sites, followed by recruitment of mitochondria to these sites. The mitochondria promote branching through ATP generation and the determination of localized hot spots of active axonal mRNA translation, which contribute to actin-dependent aspects of branching. In contrast, mitochondria do not have a role in the regulation of the microtubule cytoskeleton during NGF-induced branching. Collectively, these observations indicate that sensory axons exhibit multiple potential sites of translation, defined by presence of translational machinery, but active translation occurs following the stalling and respiration of mitochondria at these potential sites of translation. This study reveals a local role for axonal mitochondria in the regulation of the actin cytoskeleton and axonal mRNA translation underlying branching.
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Details
- Title
- Mitochondria coordinate sites of axon branching through localized intra-axonal protein synthesis
- Creators
- Mirela Spillane - Drexel UniversityAndrea Ketschek - Department of Anatomy and Cell Biology, Shriners Hospitals Pediatric Research Center, Temple University, 3500 North Broad Street, Philadelphia, PA 19140, USATanuja T Merianda - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19210, USAJeffery L Twiss - University of South CarolinaGianluca Gallo - Drexel University
- Publication Details
- Cell reports (Cambridge), v 5(6), pp 1564-1575
- Publisher
- Elsevier
- Number of pages
- 12
- Grant note
- R01 NS078030 / NINDS NIH HHS R01-NS078030 / NINDS NIH HHS R01 NS041596 / NINDS NIH HHS R01-NS041596 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000331153100009
- Scopus ID
- 2-s2.0-84890982921
- Other Identifier
- 991021892011304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology