Journal article
Mitochondrial Inorganic Polyphosphate (polyP) Is a Potent Regulator of Mammalian Bioenergetics in SH-SY5Y Cells: A Proteomics and Metabolomics Study
Frontiers in cell and developmental biology, v 10, p833127
17 Feb 2022
PMID: 35252194
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Inorganic polyphosphate (polyP) is an ancient, ubiquitous, and well-conserved polymer which is present in all the studied organisms. It is formed by individual subunits of orthophosphate which are linked by structurally similar bonds and isoenergetic to those found in ATP. While the metabolism and the physiological roles of polyP have already been described in some organisms, including bacteria and yeast, the exact role of this polymer in mammalian physiology still remains poorly understood. In these organisms, polyP shows a co-localization with mitochondria, and its role as a key regulator of the stress responses, including the maintenance of appropriate bioenergetics, has already been demonstrated by our group and others. Here, using Wild-type (Wt) and MitoPPX (cells enzymatically depleted of mitochondrial polyP) SH-SY5Y cells, we have conducted a comprehensive study of the status of cellular physiology, using proteomics and metabolomics approaches. Our results suggest a clear dysregulation of mitochondrial physiology, especially of bioenergetics, in MitoPPX cells when compared with Wt cells. Moreover, the effects induced by the enzymatic depletion of polyP are similar to those present in the mitochondrial dysfunction that is observed in neurodegenerative disorders and in neuronal aging. Based on our findings, the metabolism of mitochondrial polyP could be a valid and innovative pharmacological target in these conditions.
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Details
- Title
- Mitochondrial Inorganic Polyphosphate (polyP) Is a Potent Regulator of Mammalian Bioenergetics in SH-SY5Y Cells: A Proteomics and Metabolomics Study
- Creators
- Mariona Guitart-Mampel - Rutgers, The State University of New JerseyPedro Urquiza - Rutgers, The State University of New JerseyFausto Carnevale Neto - , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,James R. Anderson - University of LiverpoolVedangi Hambardikar - Rutgers, The State University of New JerseyErnest R. Scoma - Rutgers, The State University of New JerseyGennifer E. Merrihew - University of WashingtonLu Wang - University of WashingtonMichael J. MacCoss - University of WashingtonDaniel Raftery - University of WashingtonMandy J. Peffers - University of LiverpoolMaria E. Solesio - Rutgers, The State University of New Jersey
- Publication Details
- Frontiers in cell and developmental biology, v 10, p833127
- Publisher
- Frontiers Media S.A
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000771814000001
- Scopus ID
- 2-s2.0-85125711326
- Other Identifier
- 991022197451104721
UN Sustainable Development Goals (SDGs)
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology
- Developmental Biology