Journal article
Mitochondrial RNA polymerase is an essential enzyme in erythrocytic stages of Plasmodium falciparum
Molecular and biochemical parasitology, v 185(1), pp 48-51
Sep 2012
PMID: 22640832
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have shown that transgenic
Plasmodium falciparum
parasites expressing the yeast DHODH (dihydroorotate dehydrogenase) are independent of the mtETC (mitochondrial electron transport chain), suggesting that they might not need the mitochondrial genome (mtDNA), since it only encodes three protein subunits belonging to the mtETC and fragmentary ribosomal RNA molecules. Disrupting the mitochondrial RNA polymerase (mtRNAP), which is critical for mtDNA replication and transcription, might then cause the generation of a ρ
0
parasite line lacking mtDNA. We made multiple attempts to disrupt the mtRNAP gene by double crossover recombination methods in parasite lines expressing yDHODH either episomally or integrated in the genome, but were unable to produce the desired knockout. We verified that the mtRNAP gene was accessible to recombination by successfully integrating a triple HA tag at the 3’ end via single cross-over recombination. These studies suggest that mtRNAP is essential even in mtETC-independent
P. falciparum
parasites.
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Details
- Title
- Mitochondrial RNA polymerase is an essential enzyme in erythrocytic stages of Plasmodium falciparum
- Creators
- Hangjun Ke - Center for Molecular Parasitology and Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USAJoanne Morrisey - Center for Molecular Parasitology and Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USASuresh M Ganesan - Center for Molecular Parasitology and Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USAMichael W Mather - Center for Molecular Parasitology and Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USAAkhil B Vaidya - Center for Molecular Parasitology and Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USA
- Publication Details
- Molecular and biochemical parasitology, v 185(1), pp 48-51
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000307420700006
- Scopus ID
- 2-s2.0-84864283070
- Other Identifier
- 991014877652104721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Parasitology