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Mitochondrial responses to organelle-specific drug delivering nanoparticles composed of polypeptide and peptide complexes
Journal article   Peer reviewed

Mitochondrial responses to organelle-specific drug delivering nanoparticles composed of polypeptide and peptide complexes

Carleigh Charles, Ifat Cohen-Erez, Blake Kazaoka, Olga Melnikov, David E. Stein, Richard Sensenig, Hanna Rapaport and Zulfiya Orynbayeva
Nanomedicine (London, England), v 15(30), pp 2917-2932
01 Oct 2020
PMID: 33241963

Abstract

Biotechnology & Applied Microbiology Life Sciences & Biomedicine Nanoscience & Nanotechnology Science & Technology Science & Technology - Other Topics
Aims: The mechanistic study of the drug carrier-target interactions of mitochondria-unique nanoparticles composed of polypeptide-peptide complexes (mPoP-NPs). Materials & methods: The isolated organelles were employed to address the direct effects of mPoP-NPs on dynamic structure and functional wellbeing of mitochondria. Mitochondria morphology, respiration, membrane potential, reactive oxygen species generation, were examined by confocal microscopy, flow cytometry and oxygraphy. Lonidamine-encapsulated formulation was assessed to evaluate the drug delivery capacity of the naive nanoparticles. Results: The mPoP-NPs do not alter mitochondria structure and performance upon docking to organelles, while successfully delivering drug that causes organelle dysfunction. Conclusion: The study gives insight into interactions of mPoP-NPs with mitochondria and provides substantial support for consideration of designed nanoparticles as biocompatible and efficient mitochondria-targeted platforms.

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Collaboration types
Domestic collaboration
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Web of Science research areas
Biotechnology & Applied Microbiology
Nanoscience & Nanotechnology
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