Journal article
Mitotic Motor KIFC1 Is an Organizer of Microtubules in the Axon
The Journal of neuroscience, v 39(20), pp 3792-3811
15 May 2019
PMID: 30804089
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
KIFC1 (also called HSET or kinesin-14a) is best known as a multifunctional motor protein essential for mitosis. The present studies are the first to explore KIFC1 in terminally postmitotic neurons. Using RNA interference to partially deplete KIFC1 from rat neurons (from animals of either gender) in culture, pharmacologic agents that inhibit KIFC1, and expression of mutant KIFC1 constructs, we demonstrate critical roles for KIFC1 in regulating axonal growth and retraction as well as growth cone morphology. Experimental manipulations of KIFC1 elicit morphological changes in the axon as well as changes in the organization, distribution, and polarity orientation of its microtubules. Together, the results indicate a mechanism by which KIFC1 binds to microtubules in the axon and slides them into alignment in an ATP-dependent fashion and then cross-links them in an ATP-independent fashion to oppose their subsequent sliding by other motors.
Here, we establish that KIFC1, a molecular motor well characterized in mitosis, is robustly expressed in neurons, where it has profound influence on the organization of microtubules in a number of different functional contexts. KIFC1 may help answer long-standing questions in cellular neuroscience such as, mechanistically, how growth cones stall and how axonal microtubules resist forces that would otherwise cause the axon to retract. Knowledge about KIFC1 may help researchers to devise strategies for treating disorders of the nervous system involving axonal retraction given that KIFC1 is expressed in adult neurons as well as developing neurons.
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Details
- Title
- Mitotic Motor KIFC1 Is an Organizer of Microtubules in the Axon
- Creators
- Hemalatha Muralidharan - Drexel University College of Medicine, Department of Neurobiology and Anatomy, Philadelphia, Pennsylvania 19129Peter W Baas - Drexel University College of Medicine, Department of Neurobiology and Anatomy, Philadelphia, Pennsylvania 19129 pwb22@drexel.edu
- Publication Details
- The Journal of neuroscience, v 39(20), pp 3792-3811
- Publisher
- Society for Neuroscience; United States
- Grant note
- R01 NS028785 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000467902800002
- Scopus ID
- 2-s2.0-85066869608
- Other Identifier
- 991014878548204721
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- Web of Science research areas
- Neurosciences