Journal article
Model protein detection using antibody-immobilized tapered fiber optic biosensors (TFOBS) in a flow cell at 1310 nm and 1550 nm
Sensors and actuators. B, Chemical, v 129(2), pp 716-725
2008
Abstract
In a recent study, we reported for the first time the detection of a model protein Bovine Serum Albumin (BSA) using antibody-immobilized tapered fiber optic biosensors (TFOBS) at 1550
nm under stagnant condition, with a detection limit of 10
fg/mL. In this study, we examine the detection of BSA in a flow cell configuration because flow reduces non-specific adsorption of contaminating proteins, eliminates transmission changes due to mechanical movements, and allows for quick switching between samples.
TFOBS were fabricated with waist diameters of 5–10
μm and total lengths of 1000–1200
μm. They were housed in a specially constructed holder which served as a flow cell. The non-specific response of the TFOBS was measured by flowing in aqueous glucose solutions of various refractive indices at 0.5
mL/min. Detection experiments were conducted by immobilizing antibody to BSA on TFOBS surface, and then exposing them to 1
pg/mL–10
ng/mL of BSA at 0.5
mL/min and measuring the transmission at 1310
nm and 1550
nm. In addition, 1
pg/mL of BSA in the presence of 1
pg/mL of Ovalbumin (OVA) was measured successfully in the same apparatus.
The transmission of TFOBS is a function of refractive index of the liquid surrounding the taper, and depends on the wavelength. Both pure BSA and BSA in the presence of OVA were detected in the flow cell arrangement. The transmission response and attachment rates were quantified and found to be similar for the two cases.
Metrics
Details
- Title
- Model protein detection using antibody-immobilized tapered fiber optic biosensors (TFOBS) in a flow cell at 1310 nm and 1550 nm
- Creators
- Angela Leung - Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA 19104, United StatesP. Mohana Shankar - Department of Electrical and Computer Engineering, Drexel University, Philadelphia, PA 19104, United StatesRaj Mutharasan - Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA 19104, United States
- Publication Details
- Sensors and actuators. B, Chemical, v 129(2), pp 716-725
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering; Chemical and Biological Engineering
- Web of Science ID
- WOS:000253734300033
- Scopus ID
- 2-s2.0-38949154847
- Other Identifier
- 991014878218404721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical
- Electrochemistry
- Instruments & Instrumentation