Journal article
Modeling HIV-1 infection of differentiating populations of CD34+ progenitor cells
Journal of neurovirology, Vol.12, pp.7-7
01 May 2006
Abstract
Previous studies have suggested that the bone marrow compartment may play an integral role in the pathologic events associated with HIV-1 dementia (HIVD). Interestingly, CD34+/CD38- progenitor cells within the bone marrow are refractile to HIV-1 infection, possibly due to their low level expression of HIV-1 co-receptors, CXCR4 and CCR5, which upon differentiation are upregulated, potentially increasing susceptibility to infection. The CD34+/CD38+ TF-1 bone marrow progenitor cell line was selected as a model to study HIV-1 infection during the differentiation of hematopoietic progenitor cells. TF-1 cells were treated with a number of metabolic activators including PMA, conditioned media from PMA-treated cells, as well as cytokines such as GM-CSF, M-CSF, IL-1beta, TNF-alpha, IL-4 and their maturation was monitored through their expression of surface markers by flow cytometry. Interestingly, IL-1beta, alone or in combination with TNF-alpha leads to CXCR4 and CCR5 upregulation and preservation of CD4 expression providing a window of opportunity for HIV-1 infection to occur. Moreover, transient and stable transfection analysis demonstrated that the HIV-1 LTR activity was significantly increased following treatment of TF-1 cells with IL-1beta and conditioned media. These results indicate that progenitor cell differentiation potentially increases HIV-1 susceptibility and leads to altered LTR activity, viral transcription, and possibly productive replication.
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Details
- Title
- Modeling HIV-1 infection of differentiating populations of CD34+ progenitor cells
- Creators
- A BanerjeeA AlexakiM NonnemacherE KilareskiB Wigdahl
- Publication Details
- Journal of neurovirology, Vol.12, pp.7-7
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019170615204721