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Modeling the influence of superoxide dismutase on superoxide and nitric oxide interactions, including reversible inhibition of oxygen consumption
Journal article   Peer reviewed

Modeling the influence of superoxide dismutase on superoxide and nitric oxide interactions, including reversible inhibition of oxygen consumption

Donald G Buerk, Kathleen Lamkin-Kennard and Dov Jaron
Free radical biology & medicine, v 34(11), pp 1488-1503
01 Jun 2003
PMID: 12757859

Abstract

Free Radical Scavengers - pharmacology Models, Theoretical Blood Vessels - metabolism Humans Enzyme Inhibitors - pharmacology Oxygen Consumption Nitrites - metabolism Nitric Oxide - physiology Arteries - drug effects Nitric Oxide Synthase Type II Arteries - metabolism Hydrogen Peroxide - metabolism Nitrates - metabolism Computer Simulation Peroxynitrous Acid - metabolism Superoxides - metabolism Free Radicals - metabolism Nitric Oxide Synthase - metabolism Superoxide Dismutase - metabolism
A mathematical mass transport model was constructed in cylindrical geometry to follow coupled biochemical reactions and diffusion of oxygen, nitric oxide, superoxide, peroxynitrite, hydrogen peroxide, nitrite, and nitrate around a blood vessel. Computer simulations were performed for a 50 microm internal diameter arteriole to characterize mass transport in five concentric regions (blood, plasma layer, endothelium, vascular wall, perivascular tissue). Steady state gradients in nitric oxide, oxygen partial pressure, superoxide, and peroxynitrite, and associated production of hydrogen peroxide, nitrite, and nitrate were predicted for varying superoxide production rates, superoxide dismutase concentrations, and other physiological conditions. The model quantifies how competition between superoxide scavenging by nitric oxide and superoxide dismutase catalyzed removal varies spatially. Reversible inhibition of oxygen consumption by nitric oxide, which causes increased tissue oxygenation at deeper locations, was also included in the model. The mass transport model provides insight into complex interactions between reactive oxygen and nitrogen species in blood and tissue, and provides an objective way to evaluate the relative influence of different biochemical pathways on these interactions.

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Collaboration types
Domestic collaboration
Web of Science research areas
Biochemistry & Molecular Biology
Endocrinology & Metabolism
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