Journal article
Modulated excitation of singly ligated carboxyhemoglobin
Biophysical journal, v 65(5), pp 2059-2067
Nov 1993
PMID: 8298035
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have extended the method of modulated excitation, a small perturbation kinetic method, to study ligand binding and conformational change of hemoglobin tetramers with a single ligand bound. To restrict the excitation to the first ligand, only 1% of the hemes have bound CO, and the remainder are kept unliganded. A detailed theory is presented which agrees well with the experimental observations. This method of observing ligand recombination also provides a novel and simple method for determination of hemoglobin concentration. Additional relaxation processes are also observed. By fitting independently determined spectra to the spectra associated with the relaxations, these processes are assigned as thermal excitation and thermally driven protonation/deprotonation reactions. These added relaxations arise from the deoxy-Hb portion of the samples, and demonstrate that modulated excitation can be used effectively for temperature perturbation in the absence of photodissociation. The spectra observed are not well described by the spectra of allosteric change, however, and we conclude that there is no significant mixing of quaternary states at the first ligation step. In an appendix we present a derivation of the particular features seen in thermally modulated protonation reactions.
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Details
- Title
- Modulated excitation of singly ligated carboxyhemoglobin
- Creators
- D Liao - Department of Physics and Atmospheric Science, Drexel University, Philadelphia, Pennsylvania 19104J Jiang - Department of Physics and Atmospheric Science, Drexel University, Philadelphia, Pennsylvania 19104M Zhao - Department of Physics and Atmospheric Science, Drexel University, Philadelphia, Pennsylvania 19104F A Ferrone - Department of Physics and Atmospheric Science, Drexel University, Philadelphia, Pennsylvania 19104
- Publication Details
- Biophysical journal, v 65(5), pp 2059-2067
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Physics
- Web of Science ID
- WOS:A1993MF32100037
- Scopus ID
- 2-s2.0-0027362732
- Other Identifier
- 991014878136004721
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- Web of Science research areas
- Biophysics