Journal article
Modulation of Aortic and Cardiac G Protein Alpha Subunits and Their mRNAs during Norepinephrine Infusion in Rats
Journal of vascular research, v 32(1), pp 16-23
1995
PMID: 7873705
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
These studies examine the possibility that alterations in the expression of G protein α subunits occur during desensitization of adrenergic responses in the cardiovascular system. To desensitize adrenergic receptors, rats were infused with norepinephrine (NE) subcutaneously (0.1 mg/kg/h) for 3 or 6 days using osmotic minipumps. G protein α subunits and their mRNAs were then measured in the aorta and heart using selective antibodies and cDNA probes. Infusion of NE for 6 days significantly decreases the levels of Gsα, Giα and Goα in the aorta. The mRNAs for the α subunits are not altered in the aorta after NE infusion for 3 or 6 days indicating that reduced mRNA expression does not account for the decreased proteins. In the atrium and ventricle the levels of Gsα decrease after NE infusion for 3 days but then return to control levels by day 6. The levels of atrial and ventricular Giα are unaltered after NE infusion for 3 days but increase significantly by day 6. Goα levels do not change in the atrium or ventricle on either day. The level of Gi2α mRNA increases after NE infusion for 6 days and may account for the increased αi protein. The levels of the other Gα mRNAs do not change in the atrium or ventricle. These results demonstrate that expression of G protein α subunits is altered during cardiovascular desensitization, raising the possibility that modulation of the α subunits may contribute to reduced adrenergic responsiveness.
Metrics
Details
- Title
- Modulation of Aortic and Cardiac G Protein Alpha Subunits and Their mRNAs during Norepinephrine Infusion in Rats
- Creators
- Yuanguo Zhou - Drexel UniversityEitan FriedmanJay RobertsMark D JohnsonJames R Roberts - Emergency Medicine
- Publication Details
- Journal of vascular research, v 32(1), pp 16-23
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Emergency Medicine
- Web of Science ID
- WOS:A1995QN42600002
- Scopus ID
- 2-s2.0-0028962922
- Other Identifier
- 991019184066004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Peripheral Vascular Disease
- Physiology