Journal article
Modulation of basal expression of the human α1(I) procollagen gene (COL1A1) by tandem NF-1/Sp1 promoter elements in normal human dermal fibroblasts
Matrix biology, v 17(6), pp 425-434
1998
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Abstract
We previously demonstrated that a segment of the human α1 type I procollagen gene (COL1A1) promoter encompassing nt −174 to −84 is responsible for the highest transcriptional activity in collagen producing cells
in vitro. Here, we identified two almost identical tandem NF-1/Sp1 binding sites located between nt −129 to −107 (distal element) and nt −104 to −77 (proximal element) that are responsible for the basal regulation of COL1A1 transcription in normal human dermal fibroblasts. Transient transfection studies revealed that 8.5% of the basal COL1A1 promoter activity resides within the distal element; however, site-directed mutagenesis within the CCAAT motif in the proximal element resulted in a 98% decrease of the COL1A1 promoter activity. We conclude that each of the NF-1/Sp1 tandem binding sites has a different function. The distal element drives the transcriptional activity of the COL1A1 promoter but is not sufficient for its basal expression, whereas the NF-1 binding site in the proximal element is essential for
in vitro COL1A1 gene transcription.
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Details
- Title
- Modulation of basal expression of the human α1(I) procollagen gene (COL1A1) by tandem NF-1/Sp1 promoter elements in normal human dermal fibroblasts
- Creators
- Carol M. Artlett - Thomas Jefferson UniversityShu-Jen Chen - University of Illinois ChicagoJohn Varga - University of Illinois ChicagoSergio A. Jimenez - Thomas Jefferson University
- Publication Details
- Matrix biology, v 17(6), pp 425-434
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000076880400003
- Scopus ID
- 2-s2.0-0031773665
- Other Identifier
- 991020202229604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology