Journal article
Molecular Adhesion between Cartilage Extracellular Matrix Macromolecules
Biomacromolecules, v 15(3), pp 772-780
01 Mar 2014
PMID: 24491174
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In this study, we investigated the molecular adhesion between the major constituents of cartilage extracellular matrix, namely, the highly negatively charged proteoglycan aggrecan and the type II/IX/XI fibrillar collagen network, in simulated physiological conditions. Colloidal force spectroscopy was applied to measure the maximum adhesion force and total adhesion energy between aggrecan end-attached spherical tips (end radius R approximate to 2.5 mu m) and trypsin-treated cartilage disks with undamaged collagen networks. Studies were carried out in various aqueous solutions to reveal the physical factors that govern aggrecan-collagen adhesion. Increasing both ionic strength and [Ca2+] significantly increased adhesion, highlighting the importance of electrostatic repulsion and Ca2+-mediated ion bridging effects. In addition, we probed how partial enzymatic degradation of the collagen network, which simulates osteoarthritic conditions, affects the aggrecan-collagen interactions. Interestingly, we found a significant increase in aggrecan-collagen adhesion even when there were no detectable changes at the macro- or microscales. It is hypothesized that the aggrecan-collagen adhesion, together with aggrecan-aggrecan self-adhesion, works synergistically to determine the local molecular deformability and energy dissipation of the cartilage matrix, in turn, affecting its macroscopic tissue properties.
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Details
- Title
- Molecular Adhesion between Cartilage Extracellular Matrix Macromolecules
- Creators
- Fredrick P. Rojas - Massachusetts Institute of TechnologyMichael A. Batista - MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USAC. Alexander Lindburg - Clemson UniversityDelphine Dean - Clemson UniversityAlan J. Grodzinsky - MIT, Dept Mech Engn, Cambridge, MA 02139 USAChristine Ortiz - MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USALin Han - MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA
- Publication Details
- Biomacromolecules, v 15(3), pp 772-780
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 9
- Grant note
- AR60331 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA CMMI-0758651 / National Science Foundation; National Science Foundation (NSF) Institute for Soldier Nanotechnologies at MIT through the U.S. Army Research Office Faculty Start-up Grant at Drexel University Shriners of North America N00244-09-1-0064 / National Security Science and Engineering Faculty Fellowship R01AR060331 / NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000332756600009
- Scopus ID
- 2-s2.0-84896783730
- Other Identifier
- 991019169554204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Organic
- Polymer Science