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Journal article
Molecular Analysis of the DNA Sequences Involved in the Transcriptional Regulation of the Phosphate-Repressible Acid Phosphatase Gene (PHO5) of Saccharomyces cerevisiae
Proceedings of the National Academy of Sciences - PNAS, v 83(16), pp 6070-6074
01 Aug 1986
PMID: 3526349
Abstract
The expression of the PHO5 gene of Saccharomyces cerevisiae is transcriptionally regulated in response to the level of inorganic phosphate present in the growth medium. We have identified, by DNA deletion analysis, the sequences (upstream activator sequences) that mediate this response. The sequence 5′ CTGCACAATAG 3′ is present in two copies located within a 60-base-pair region. The presence of a single copy of the sequence is sufficient for the phosphate-mediated transcriptional response. In addition, a DNA fragment that contains two copies of this sequence will act to repress transcription of a CYC1-lacZ fusion when placed either upstream or downstream of the CYC1 activator sequence.
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Details
- Title
- Molecular Analysis of the DNA Sequences Involved in the Transcriptional Regulation of the Phosphate-Repressible Acid Phosphatase Gene (PHO5) of Saccharomyces cerevisiae
- Creators
- Lawrence W. BergmanDiana C. McClintonStephen L. MaddenLisa H. Preis
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 83(16), pp 6070-6074
- Publisher
- National Academy of Sciences of the United States of America
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1986D754900069
- Scopus ID
- 2-s2.0-0347920500
- Other Identifier
- 991020547450804721
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- Web of Science research areas
- Biochemistry & Molecular Biology