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Molecular analysis of the PHO81 gene of Saccharomyces cerevisiae
Journal article   Open access   Peer reviewed

Molecular analysis of the PHO81 gene of Saccharomyces cerevisiae

C L Creasy, S L Madden and L W Bergman
Nucleic acids research, v 21(8), pp 1975-1982
25 Apr 1993
DOI: https://doi.org/10.1093/nar/21.8.1975
PMID: 8493108
url
https://doi.org/10.1093/nar/21.8.1975View
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Abstract

Gene Expression Regulation, Fungal Promoter Regions, Genetic Saccharomyces cerevisiae - genetics Repressor Proteins Molecular Sequence Data Fungal Proteins - genetics DNA, Fungal RNA, Messenger - metabolism Restriction Mapping Cyclins Blotting, Northern Base Sequence Cloning, Molecular Saccharomyces cerevisiae Proteins Genes, Fungal Cyclin-Dependent Kinases Mutation
The PHO81 gene product is a positive regulatory factor required for the synthesis of the phosphate repressible acid phosphatase (encoded by the PHO5 gene) in Saccharomyces cerevisiae. Genetic analysis has suggested that PHO81 may be the signal acceptor molecule; however, the biochemical function of the PHO81 gene product is not known. We have cloned the PHO81 gene and sequenced the promoter. A PHO81-LacZ fusion was shown to be a valid reporter since its expression is regulated by the level of inorganic phosphate and is controlled by the same regulatory factors that regulate PHO5 expression. To elucidate the mechanism by which PHO81 functions, we have isolated and cloned dominant mutations in the PHO81 gene which confer constitutive synthesis of acid phosphatase. We have demonstrated that overexpression of the negative regulatory factor, PHO80, but not the negative regulatory factor PHO85, partially blocks the constitutive acid phosphatase synthesis in a strain containing a dominant constitutive allele of PHO81. This suggests that PHO81 may function by interacting with PHO80 or that these molecules compete for the same target.

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Domestic collaboration
Web of Science research areas
Biochemistry & Molecular Biology
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