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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Molecular basis for interactions between an acyl carrier protein and a ketosynthase
Nature chemical biology, v 15(7), pp 669-671
01 Jul 2019
PMID: 31209348
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Fatty acid synthases are dynamic ensembles of enzymes that can biosynthesize long hydrocarbon chains efficiently. Here we visualize the interaction between the Escherichia coli acyl carrier protein (AcpP) and beta-ketoacyl-ACP-synthase I (FabB) using X-ray crystallography, NMR, and molecular dynamics simulations. We leveraged this structural information to alter lipid profiles in vivo and provide a molecular basis for how protein-protein interactions can regulate the fatty acid profile in E. coli.
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Details
- Title
- Molecular basis for interactions between an acyl carrier protein and a ketosynthase
- Creators
- Jacob C. Milligan - University of California, IrvineD. John Lee - Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USADavid R. Jackson - Univ Calif Irvine, Dept Chem, Irvine, CA 92717 USAAndrew J. Schaub - University of California, IrvineJoris Beld - University of California San DiegoJesus F. Barajas - Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92717 USAJoseph J. Hale - Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USARay Luo - University of California, IrvineMichael D. Burkart - University of California San DiegoShiou-Chuan Tsai - University of California, Irvine
- Publication Details
- Nature chemical biology, v 15(7), pp 669-671
- Publisher
- Springer Nature
- Number of pages
- 5
- Grant note
- DE-AC02-05CH11231 / Department of Energy (DOE) Office of Science User Facility; United States Department of Energy (DOE) T32GM108561 / institutional Chemical and Structural Biology Training Grant (National Institute of General Medical Sciences) 1516156 / National Science Foundation (NSF) Division of Integrative Organismal Systems (IOS); National Science Foundation (NSF) GM093040; GM079383; GM100305; GM095970 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA National Science Foundation Graduate Research Fellowship; National Science Foundation (NSF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000472625600010
- Scopus ID
- 2-s2.0-85067854293
- Other Identifier
- 991020535070904721
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- Web of Science research areas
- Biochemistry & Molecular Biology